FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19934257
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG3353.2196112E-108.7768655
TACACTG32350.08.6560675
TAGACTG23550.08.6206885
TAGGACC20200.08.5253914
TAGACAG33600.08.5007215
TACACCG9150.08.4160155
TATACTG23400.08.3767795
TAATACT23100.08.182434
CTAGGAC26450.08.0989053
TAGGACA22850.07.9043114
TCTATAC15950.07.90027763
GTGTAAG25500.07.7972481
TACACAG40900.07.77083065
CTAATAC19700.07.7467733
CTAGACT24100.07.72671944
AGGACCG23200.07.72479345
GTATAGA16550.07.69904041
TCTACAC25250.07.65205673
ATAGGAC16050.07.58935263
CGTCAGA29950.07.57375761
Produced by FastQC (version 0.11.5)