FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004948999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004948999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19934257
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC19850.09.3102484
TAGACTG25400.09.039855
CTAGGAC26700.09.0190023
TACACTG31900.08.6462275
CTAGACT22500.08.2137084
ATACCGT5300.08.1891776
GTCTAAG19700.08.0348361
CCTATAC15350.08.0263043
GTCTAGG26300.07.9891451
CTTATAC15150.07.94743733
GTATAGG16700.07.8006581
GTACTAG11500.07.79555371
GTCCTAC15700.07.76219221
GTCCTAG27150.07.73902461
TGGACTG32050.07.73208055
GTGTAGG29950.07.717061
TAGGACA22050.07.68289044
CTATACT21750.07.66011954
CCTACAC28550.07.64997153
TATACTG23350.07.6149665
Produced by FastQC (version 0.11.5)