FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856648
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCGAG277107397.00435615448596No Hit
AGCTCGCG238030.8332493187820131No Hit
AGCACGAG56730.19858939568333234No Hit
AGCTCCAG55150.19305843772141335No Hit
AGCTCGAC46180.16165799916545545No Hit
AGCTCGAA46080.16130793853495426No Hit
CGCTCGAG45120.1579473564821427No Hit
AGCGCGAG34950.12234619036017037No Hit
AGCTCGAT34260.11993077200971207No Hit
AGCTCGTG33230.11632514751554969No Hit
AGCTAGAG31460.11012907435567841No Hit
ACCTCGAG31320.10963898947297672No Hit
AGATCGAG28990.10148257678229869No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)