FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158708
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT332451.0524872827751093No Hit
TGCTGCTACCGTTCTC313070.9911330835265557No Hit
AAACGACCTCTTCAAG307950.9749239245919534No Hit
TATGCTTTGGACTGTT291240.9220225484596867No Hit
ATGGGTTACAATCGAA290680.9202496717012145No Hit
GCCTCTTGATCTGTGA279310.8842539418015214No Hit
AAAGCGGACTCGGGCT270280.8556663040711583No Hit
TCCAAAGTGCTCACGG258070.8170112590337569No Hit
TAGTGCATGAACCCGC246640.7808255780527987No Hit
TAACTCACTGGTGTCC235590.7458429205865182No Hit
TGTGGCTCTTACGATC230740.7304885415176078No Hit
GCAGGTCTGGCTGTTA225650.7143743581236378No Hit
GAAGTATGAACAGGTG212960.6741997044361175No Hit
TTCCCATCTCTTTGTT204370.6470050413016968No Hit
GAGCCTGACCGAGTTT196970.6235777412790293No Hit
TCACCTGACTCAATGC194710.6164229172180524No Hit
GGAACTACTACCAAGG193950.6140168701886974No Hit
ATGTCCAGAGTCGGTC189770.6007836115272447No Hit
CTAGAGACTAATCTGC187090.5922991298974137No Hit
TGAGAATGAGCAGTTT184690.5847010866468189No Hit
TTCGTCCGACCTGTGG180040.5699798778487913No Hit
TCGTATACTGCTAATC178950.5665290998724796No Hit
TCACACAGATTGGGAG175240.5547837913476016No Hit
ATTCCTAACTGCTACA172190.5451279447166373No Hit
TCCGCGAGAGAACAGC168370.5330343925427738No Hit
ACTGATTCTCGGCTCA165470.5238534236149717No Hit
TGGCCTGGACCCGAGT163970.5191046465833499No Hit
AGGTCGAGAAGGGAGG163610.5179649400957607No Hit
TGGACCTCTAGGGAGG161340.5107784575212396No Hit
GCAATCATGGAACGTG153950.4873828160121163No Hit
CCATGATGATTCGGGC152090.48149433249290535No Hit
ACGAGGATGGAGCTGA142320.4505639647602754No Hit
TTCGATGGAAATCCTG139910.44293426299613636No Hit
TCCCAGACTGTCTTGA135090.4276748594678584No Hit
AAGGTGCGAACAGATA134030.424319057032179No Hit
TTTCGTAACAGTACGG131370.4158978924294363No Hit
TGGTCCTTGGAGAGGG127310.40304453593051337No Hit
TGCGGTGACCTTAGTG124550.3943067861923293No Hit
TCTCTCTACTCTGGAT123640.391425861459812No Hit
TCCTTCACTTGTCACA113970.36081207886262356No Hit
AGCCCAGTGAAAGTAC112100.3548919368298684No Hit
TCGCCGAGAAGGTAAT111240.3521693046650719No Hit
AGCTGCTCTTGTCACA109060.34526774871244825No Hit
TTCGATGGACAGGTGA109040.34520443168535997No Hit
TGATATCCTCGATGTT107430.34010741100475256No Hit
AACCCTCACGCGGTTC106750.3379546320837507No Hit
CTTTCGGTGCTTTGTT104750.33162292937492166No Hit
AACTCCCTGCTTCACC104360.33038824734669997No Hit
CCCTTGCCTCCTACGG101150.32022586449902934No Hit
ATGGCCGTGATACGCT100150.31706001314461485No Hit
TGTTTGTTGCAAGTTC95050.30091417123710074No Hit
CTAACTGGAACCTTGA93480.2959437846106699No Hit
TCGGCTTACCTCCTAC93270.29527895582624286No Hit
AAACACGCTGCAGTTT92450.29268295771562297No Hit
TGGCCTTTGCGAAAGC92360.29239803109372564No Hit
ACCCTTGTGGGCCATC90560.2866994986557795No Hit
GAAGTAATGAGTCTGT89340.2828371600033938No Hit
AAATAGGACTCTATCT88240.2793547235135378No Hit
TACAACGTGAGCAGGT87880.2782150170259486No Hit
ACAGCCGACAAACATG86280.27314965485888537No Hit
TCAAAGGCTCTTAGTG86080.27251648458800243No Hit
CAATGTGTGGTTGACT84770.26836921931371943No Hit
GGGGGGGGGGGGGGGG82640.26162595592881643No Hit
CTTTCGGTGAGCCATT82460.2610561026850219No Hit
GGACATTTGGCCGCTA78930.24988064740393856No Hit
TGAACTGTGTGCTGGC78270.247791185510025No Hit
CAGCCGATGTAATAGG77210.2444353830743456No Hit
AGTGTTCGATTCGCCT73900.2339564150912335No Hit
GGCTTGCGAATACGCT71070.2249970557582404No Hit
GATAAGAACATTGGTC66840.21160550452906696No Hit
GTAAAGCCTGAAATCA65940.20875623831009388No Hit
TTGGGAGGAATCTGTG65480.2072999466870632No Hit
GTGCTAATGGGAGTCA64790.20511550925251718No Hit
TGTACTGGAAATGCTC63300.20039839073443955No Hit
GGAACATCTTTACGAC61870.19587122329762677No Hit
CGACGTTCTTGCTACA60960.19299029856510952No Hit
TCTTGAACTCGTACAT60520.19159732396916712No Hit
GGACCACTGGCTAGAG59570.18858976518247333No Hit
GGGTGAAACCGACTAT57650.18251133058199745No Hit
AGGGACCCTAATCCTG57500.18203645287883527No Hit
TAACGAGCTTACTTTC56640.17931382071403879No Hit
TACCACCTGAAACATG56430.17864899192961173No Hit
TACGTGTGAAACGCAG55710.17636957895443328No Hit
CGGATGAGAACAGGCA55590.17598967679190353No Hit
AGCTTGTGAACCGTGC55070.17434343408760797No Hit
CATGATGTGCCTAGTA53500.16937304746117715No Hit
AACTAGCACTGCCACG52850.16731524408080772No Hit
ACTTATCTGTCAGGTT52290.16554236732233557No Hit
TCGGACCGAATGTCCA51900.1643076852941139No Hit
ACGTGTTGATCTTTGT51570.16326295434715712No Hit
TGGTAGGGATGTGCTA48980.1550633993392235No Hit
GTCCGAGTGGTCTTGA48930.1549051067715028No Hit
AACTACGACATTGCCA48750.15433525352770816No Hit
GTAGATAGAGCTGAGA47750.15116940217329364No Hit
TTTAGGGACTGGGCAA47320.14980808609089538No Hit
CGTCGTACTTGCGCCA47120.14917491582001247No Hit
CTCGGATCTAGTACGG46950.14863672108976203No Hit
AACGAACCTGACACCC46730.1479402337917908No Hit
AGTTTGCGAGCAGGAT46110.1459774059520538No Hit
TACCACCTGTACTTTC45680.14461608986965557No Hit
GGTAGTTTGTCCAGCG45470.14395126108522852No Hit
TGAGGAGTGGTCCCAG44790.14179848216422664No Hit
GCCATAATGACCGTTA44430.1406587756766374No Hit
CCTTCAGTGCAACGCC44040.13942409364841576No Hit
CTTTAACTGTTCGCCT43610.13806277756601748No Hit
CAGGCCATGTCGTAGT41010.12983156404453972No Hit
CGATCGGTGCGAGGAT40940.1296099544497307No Hit
CTTACATTGGAAGGCT40170.12717224890683151No Hit
CAAAGACCTGCTTTGA39790.125969225392154No Hit
AGAACGCGACACAGTT39610.1253993721483594No Hit
GAGAAGAGATGCTACA39560.12524107958063868No Hit
CCGTTACTGTTGGTTG39470.12495615295874135No Hit
GTCCGTTACGGATGGT39080.1237214709305197No Hit
TGCAATGACTCCGTAA38700.12251844741584218No Hit
GGGATGTGATGCATGT37620.11909932795307449No Hit
GAAAGTCCTTATCTGG37290.11805459700611769No Hit
GAACCATACTTTGGGT37220.11783298741130867No Hit
AACTACGACGCTGTTA37180.11770635335713209No Hit
TCCTCATCTTTCTGGA36980.11707318308624919No Hit
GGCTTAAACTGCATCA36100.1142872338943644No Hit
GCTGGACACATTGGTC35770.1132425029474076No Hit
TCCGTAAGATTACGCA35500.1123877230817157No Hit
AATTCTGCTTAGCGAG35270.11165957727020034No Hit
ATGGCCGTGGGCCAAA35110.11115304105349401No Hit
ACAAGACCTAAGCTAA34700.10985504199818408No Hit
CCTGAGGACTGACTCA34620.1096017738898309No Hit
CCCACCTCTTGACCTA34580.10947513983565432No Hit
TGTCTTCCTGAAATCA34190.10824045780743266No Hit
TCGTAGAACCGCACTG34070.1078605556449029No Hit
TATGGGTACCCTTTGC33190.10507460645301812No Hit
ATAGCCAACATCTGTG32720.10358665631644332No Hit
AGGTGATCTGTTAGTC32420.10263690091011896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)