FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626540
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT393031.0837602783920761No Hit
TGCTGCTACCGTTCTC373651.0303209119436156No Hit
AAACGACCTCTTCAAG361990.9981690536985668No Hit
ATGGGTTACAATCGAA358540.9886558537890109No Hit
TATGCTTTGGACTGTT355680.9807695489364517No Hit
GCAGGTCTGGCTGTTA328830.9067320365968664No Hit
AAAGCGGACTCGGGCT322290.888698318507448No Hit
TCCAAAGTGCTCACGG307580.8481362400525019No Hit
GAAGTATGAACAGGTG290200.8002117721023345No Hit
TAGTGCATGAACCCGC284650.7849079287695709No Hit
TGTGGCTCTTACGATC273000.7527836450170134No Hit
TAACTCACTGGTGTCC268760.741092060200632No Hit
TTCCCATCTCTTTGTT249040.6867151610074617No Hit
GCCTCTTGATCTGTGA239850.6613742024078046No Hit
TCACCTGACTCAATGC239720.6610157340054156No Hit
TGAGAATGAGCAGTTT234240.6459049121200924No Hit
GGAACTACTACCAAGG226060.6233489772620735No Hit
GAAGTAATGAGTCTGT218970.6037986620856244No Hit
CTAGAGACTAATCTGC216750.5976771247525189No Hit
TCGTATACTGCTAATC215710.5948093775334065No Hit
ATGTCCAGAGTCGGTC212960.5872263920982534No Hit
ATTCCTAACTGCTACA209250.5769962553839196No Hit
TTCGTCCGACCTGTGG208840.5758657011917695No Hit
TCACACAGATTGGGAG206980.5707368455883569No Hit
TGCGGTGACCTTAGTG206690.5699371853061045No Hit
CCATGATGATTCGGGC205560.5668212676545689No Hit
AGGTCGAGAAGGGAGG203860.5621336039310196No Hit
TCCGCGAGAGAACAGC200160.5519310417091774No Hit
ACTGATTCTCGGCTCA198130.5463334197334098No Hit
TGGACCTCTAGGGAGG191280.5274448923767558No Hit
GCAATCATGGAACGTG188130.5187589272419442No Hit
TGGCCTGGACCCGAGT180080.4965614607863142No Hit
TTCGATGGAAATCCTG168830.46554015673341537No Hit
ACGAGGATGGAGCTGA164890.4546758066917778No Hit
TTTCGTAACAGTACGG162940.44929878065594203No Hit
AAGGTGCGAACAGATA161770.44607256503444054No Hit
TGGTCCTTGGAGAGGG154750.4267152713054316No Hit
TCCCAGACTGTCTTGA153540.42337875771396427No Hit
TCTCTCTACTCTGGAT152770.4212555217921214No Hit
GCGGTTCGAGTACGTT146780.4047384007897335No Hit
TTCGATGGACAGGTGA138600.3821824659317145No Hit
TCGCCGAGAAGGTAAT135340.3731931813794967No Hit
AGCTGCTCTTGTCACA132790.36616168579417296No Hit
TCCTTCACTTGTCACA132570.3655550469593607No Hit
TGATATCCTCGATGTT132570.3655550469593607No Hit
AACTCCCTGCTTCACC129060.35587640009485627No Hit
CTTTCGGTGCTTTGTT127870.35259503548837184No Hit
GGGGGGGGGGGGGGGG120820.33315501828188854No Hit
TCGGCTTACCTCCTAC120670.33274140089451654No Hit
AGCCCAGTGAAAGTAC119880.3305630159876908No Hit
AACCCTCACGCGGTTC118110.3256823308167013No Hit
CCCTTGCCTCCTACGG117770.32474479807199147No Hit
ATGGCCGTGATACGCT117470.3239175632972475No Hit
TGTTTGTTGCAAGTTC113580.31319108571806736No Hit
AAACACGCTGCAGTTT112190.3093582312617536No Hit
CAATGTGTGGTTGACT105580.29113149172489483No Hit
CTAACTGGAACCTTGA105110.28983549057779595No Hit
TACAACGTGAGCAGGT104130.28713319031363227No Hit
TGGCCTTTGCGAAAGC103530.2854787207641443No Hit
AAATAGGACTCTATCT101400.27960535386346214No Hit
TCAAAGGCTCTTAGTG101350.2794674814010048No Hit
ACCCTTGTGGGCCATC99690.27489011564742155No Hit
GGACATTTGGCCGCTA97770.2695958130890601No Hit
ACAGCCGACAAACATG97550.26898917425424784No Hit
CTTTCGGTGAGCCATT96550.2662317250051013No Hit
TGAACTGTGTGCTGGC95560.2635018502484462No Hit
CAGCCGATGTAATAGG89320.24629536693377158No Hit
GAGGTTGGAGGAATAT88900.24513723824913003No Hit
AGTGTTCGATTCGCCT86770.23926387134844784No Hit
GATAAGAACATTGGTC83460.2301367143337727No Hit
GGCTTGCGAATACGCT81680.2252284546702918No Hit
GTGCTAATGGGAGTCA80140.22098198282660608No Hit
CGACGTTCTTGCTACA77830.21461227506107747No Hit
TTGGGAGGAATCTGTG76920.2121029962443541No Hit
GTAAAGCCTGAAATCA75800.20901465308530995No Hit
GGAACATCTTTACGAC73600.20294826473718752No Hit
TGTACTGGAAATGCTC73450.20253464734981552No Hit
AGGGACCCTAATCCTG70810.1952549813320686No Hit
GGGTGAAACCGACTAT70200.19357293729008918No Hit
TACGTGTGAAACGCAG68400.18860952864162536No Hit
TAACGAGCTTACTTTC66790.18417003535049936No Hit
TACCACCTGAAACATG66660.18381156694811032No Hit
TCTTGAACTCGTACAT66300.18281888521841755No Hit
CGGATGAGAACAGGCA66300.18281888521841755No Hit
ACTTATCTGTCAGGTT65540.18072322378906616No Hit
CATGATGTGCCTAGTA65290.18003386147677952No Hit
AGCTTGTGAACCGTGC65140.17962024408940755No Hit
AACTAGCACTGCCACG59930.1652539335013539No Hit
AACTACGACATTGCCA56960.1570643092313886No Hit
ACGTGTTGATCTTTGT56870.15681613879896542No Hit
CGTCGTACTTGCGCCA56020.15447230693719083No Hit
TGGTAGGGATGTGCTA55660.15347962520749805No Hit
GTAGATAGAGCTGAGA55040.15177000667302717No Hit
AACGAACCTGACACCC54720.1508876229133003No Hit
CTCGGATCTAGTACGG54700.15083247392831733No Hit
TCGGACCGAATGTCCA54370.149922515676099No Hit
GGTAGTTTGTCCAGCG53790.14832319511159398No Hit
AGTTTGCGAGCAGGAT53120.14647570411466576No Hit
CCTTCAGTGCAACGCC52590.14501425601261808No Hit
TTTAGGGACTGGGCAA52340.14432489370033144No Hit
TACCACCTGTACTTTC50690.1397751024392396No Hit
TGAGGAGTGGTCCCAG50480.13919603809691883No Hit
CTTTAACTGTTCGCCT50090.13812063288975168No Hit
AGAACGCGACACAGTT48700.13428777843343792No Hit
GAGAAGAGATGCTACA47150.13001373209726075No Hit
GAAAGTCCTTATCTGG46910.12935194427746557No Hit
CTTACATTGGAAGGCT46330.12775262371296056No Hit
GCCATAATGACCGTTA45500.1254639408361689No Hit
CAAAGACCTGCTTTGA45380.12513304692627134No Hit
GGGATGTGATGCATGT44890.1237818967941895No Hit
CGATCGGTGCGAGGAT44610.12300981100442847No Hit
TGCAATGACTCCGTAA44200.12187925681227836No Hit
CAGGCCATGTCGTAGT43100.11884606263821715No Hit
GAAGGACACCCAGCTG43020.11862546669828543No Hit
AATTCTGCTTAGCGAG43010.11859789220579396No Hit
TCCTCATCTTTCTGGA42880.11823942380340488No Hit
AACTACGACGCTGTTA42470.1171088696112548No Hit
GGACCACTGGCTAGAG42260.11652980526893403No Hit
GCTGGACACATTGGTC42250.11650223077644256No Hit
CCGTTACTGTTGGTTG42120.11614376237405351No Hit
ATAGCCAACATCTGTG41700.11498563368941195No Hit
GCGTTTAGAAGTCTAC41340.11399295195971919No Hit
TGTCTTCCTGAAATCA40750.11236605690272271No Hit
CCCACCTCTTGACCTA40630.11203516299282512No Hit
GGCTTAAACTGCATCA40550.11181456705289339No Hit
ATGGCCGTGGGCCAAA39940.11013252301091399No Hit
TCGTAGAACCGCACTG39250.10822988302900285No Hit
ACAAGACCTAAGCTAA39250.10822988302900285No Hit
TATGGGTACCCTTTGC38710.1067408604344637No Hit
CCTGAGGACTGACTCA38680.10665813695698932No Hit
AGGTGATCTGTTAGTC38000.10478307146756964No Hit
TCCGTAAGATTACGCA37240.10268741003821825No Hit
GCGTTAATGGTACGTT36890.10172230280101695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)