FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258932
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT346091.061973677266049No Hit
TGCTGCTACCGTTCTC330971.0155781096383723No Hit
AAACGACCTCTTCAAG320310.9828680070648912No Hit
ATGGGTTACAATCGAA313930.9632910413595619No Hit
TATGCTTTGGACTGTT304860.9354598377628007No Hit
AAAGCGGACTCGGGCT286640.8795519513754813No Hit
GCCTCTTGATCTGTGA281930.8650993638406693No Hit
GCAGGTCTGGCTGTTA279180.8566610165538894No Hit
TCCAAAGTGCTCACGG268090.8226314633137481No Hit
TAGTGCATGAACCCGC260370.7989427211123155No Hit
GAAGTATGAACAGGTG245990.7548178360272629No Hit
TGTGGCTCTTACGATC242390.7437712723063875No Hit
TAACTCACTGGTGTCC242170.743096204523445No Hit
TTCCCATCTCTTTGTT218340.6699740896710947No Hit
TCACCTGACTCAATGC209910.6441067196247114No Hit
GGAACTACTACCAAGG203280.6237626314387658No Hit
GAGCCTGACCGAGTTT202480.6213078395007935No Hit
TGAGAATGAGCAGTTT198250.6083281271287649No Hit
ATGTCCAGAGTCGGTC193120.5925867738265175No Hit
CTAGAGACTAATCTGC189920.5827676060746282No Hit
TTCGTCCGACCTGTGG189760.5822766476870337No Hit
TCGTATACTGCTAATC189360.5810492517180476No Hit
GAAGTAATGAGTCTGT187500.5753418604622619No Hit
TGCGGTGACCTTAGTG187150.574267888989399No Hit
TCACACAGATTGGGAG186770.5731018628188621No Hit
TCCGCGAGAGAACAGC179960.5522054464468727No Hit
ATTCCTAACTGCTACA179720.5514690088654811No Hit
AGGTCGAGAAGGGAGG176990.5430920313771506No Hit
TGGACCTCTAGGGAGG174550.535604915966335No Hit
ACTGATTCTCGGCTCA170500.5231775317803502No Hit
TGGCCTGGACCCGAGT170460.5230547921834515No Hit
CCATGATGATTCGGGC167150.5128980905400911No Hit
GCAATCATGGAACGTG157780.4841463399665903No Hit
TTCGATGGAAATCCTG147840.45364555013728425No Hit
ACGAGGATGGAGCTGA145780.4473244608970055No Hit
AAGGTGCGAACAGATA142690.4378428270365875No Hit
TTTCGTAACAGTACGG141130.43305598275754137No Hit
TCCCAGACTGTCTTGA140180.4301409173311993No Hit
TGGTCCTTGGAGAGGG137830.42292996601340566No Hit
TCTCTCTACTCTGGAT134060.4113617590057111No Hit
TCGCCGAGAAGGTAAT121620.37318974437024155No Hit
GCGGTTCGAGTACGTT120020.3682801604942969No Hit
TCCTTCACTTGTCACA117000.3590133209284514No Hit
TTCGATGGACAGGTGA116820.3584609927424076No Hit
AGCTGCTCTTGTCACA115330.3538889427579342No Hit
TGATATCCTCGATGTT115250.35364346356413695No Hit
AGCCCAGTGAAAGTAC112240.34440730889751614No Hit
AACTCCCTGCTTCACC111700.3427503243393848No Hit
CTTTCGGTGCTTTGTT110950.34044895689753574No Hit
AACCCTCACGCGGTTC110830.3400807381068399No Hit
CCCTTGCCTCCTACGG106070.32547472607590466No Hit
ATGGCCGTGATACGCT104950.3220380173627434No Hit
TCGGCTTACCTCCTAC101010.30994816706822975No Hit
TGTTTGTTGCAAGTTC97900.30040516340936235No Hit
AAACACGCTGCAGTTT97490.2991470825411515No Hit
CTAACTGGAACCTTGA94850.29104626914584286No Hit
ACCCTTGTGGGCCATC94570.2901870919675526No Hit
GGGGGGGGGGGGGGGG94360.28954270908383484No Hit
TGGCCTTTGCGAAAGC94080.2886835319055445No Hit
TACAACGTGAGCAGGT93030.28546161748695587No Hit
CAATGTGTGGTTGACT90810.2786495698590827No Hit
AAATAGGACTCTATCT90670.2782199812699375No Hit
TCAAAGGCTCTTAGTG87650.268953141704092No Hit
ACAGCCGACAAACATG85760.2631536957506324No Hit
CTTTCGGTGAGCCATT85630.26275479206071195No Hit
TGAACTGTGTGCTGGC85030.2609136981072327No Hit
GGACATTTGGCCGCTA81010.2485783686189218No Hit
AGTGTTCGATTCGCCT78960.24228796427786772No Hit
CAGCCGATGTAATAGG78890.24207316998329514No Hit
GAGGTTGGAGGAATAT75190.2307197572701732No Hit
CGACGTTCTTGCTACA69360.21283046102219988No Hit
GTGCTAATGGGAGTCA68230.209363067409814No Hit
TTGGGAGGAATCTGTG68100.20896416371989351No Hit
TGTACTGGAAATGCTC66880.20522060601448575No Hit
GGAACATCTTTACGAC63370.19445020638663219No Hit
GGACCACTGGCTAGAG62290.19113623727036955No Hit
GGGTGAAACCGACTAT62260.1910441825726956No Hit
AGGGACCCTAATCCTG60850.18671761178201937No Hit
TCTTGAACTCGTACAT60650.1861039137975263No Hit
TAACGAGCTTACTTTC60360.18521405172001135No Hit
TACGTGTGAAACGCAG58850.1805806319370886No Hit
TACCACCTGAAACATG58530.17959871516189965No Hit
AGCTTGTGAACCGTGC58440.1793225510688778No Hit
CGGATGAGAACAGGCA58390.1791691265727545No Hit
ACTTATCTGTCAGGTT55170.16928858902241592No Hit
CATGATGTGCCTAGTA54520.16729407057281342No Hit
ACGTGTTGATCTTTGT52420.1608502417356361No Hit
GGCTTGCGAATACGCT51950.15940805147207734No Hit
TCGGACCGAATGTCCA51460.15790449141006932No Hit
GTCCGAGTGGTCTTGA50800.15587928806124215No Hit
AACTAGCACTGCCACG50500.15495874108450253No Hit
TGGTAGGGATGTGCTA50210.15406887900698757No Hit
CTCGGATCTAGTACGG49690.15247326424730556No Hit
AACGAACCTGACACCC49260.15115381358064545No Hit
GTAGATAGAGCTGAGA49050.1505094306969277No Hit
CCTTCAGTGCAACGCC48570.14903655553414433No Hit
AACTACGACATTGCCA48290.148177378355854No Hit
TACCACCTGTACTTTC48190.14787052936360748No Hit
CGTCGTACTTGCGCCA47540.14587601091400496No Hit
TTTAGGGACTGGGCAA46740.14342121897603263No Hit
GGTAGTTTGTCCAGCG46590.14296094548766283No Hit
AGTTTGCGAGCAGGAT45860.1407209478442631No Hit
GCCATAATGACCGTTA45830.14062889314658913No Hit
TGAGGAGTGGTCCCAG44890.13774451261947165No Hit
CTTTAACTGTTCGCCT44550.13670122604583343No Hit
AGAACGCGACACAGTT43290.132834928743527No Hit
GATAAGAACATTGGTC43170.13246670995283116No Hit
GGGATGTGATGCATGT41970.1287845220458727No Hit
GTCCGTTACGGATGGT41770.1281708240613796No Hit
CAGGCCATGTCGTAGT41400.12703548279006743No Hit
CGATCGGTGCGAGGAT41150.12626836030945107No Hit
CTTACATTGGAAGGCT40800.12519438883658818No Hit
GAGAAGAGATGCTACA40720.12494890964279096No Hit
GAACCATACTTTGGGT40090.12301576099163776No Hit
CAAAGACCTGCTTTGA40060.12292370629396379No Hit
CCGTTACTGTTGGTTG39610.12154288582885436No Hit
TGCAATGACTCCGTAA39380.12083713314668733No Hit
GAAAGTCCTTATCTGG38330.11761521872809864No Hit
AACTACGACGCTGTTA38220.11727768483662745No Hit
GAAGGACACCCAGCTG37690.1156513851777208No Hit
TCCTCATCTTTCTGGA37530.11516042679012634No Hit
AATTCTGCTTAGCGAG36940.11335001773587175No Hit
CCCACCTCTTGACCTA36700.11261358015448004No Hit
ATGGCCGTGGGCCAAA36370.11160097848006648No Hit
TGTCTTCCTGAAATCA36100.11077248620100082No Hit
TCCGTAAGATTACGCA35500.10893139224752157No Hit
TATGGGTACCCTTTGC34990.10736646238706422No Hit
CCTGAGGACTGACTCA34950.10724372279016561No Hit
TCGTAGAACCGCACTG34430.1056481080304836No Hit
AGGTGATCTGTTAGTC33440.10261030300724287No Hit
ACAAGACCTAAGCTAA32620.10009414127082124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)