FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16409080
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG3003.7471182E-109.3284334
TATACTG18100.09.2784245
TAGACTG20300.08.8244075
TACACTG25700.08.8217245
TAGACCG7350.08.7587485
GTATACG3753.8198777E-118.5852241
TACACCG7050.08.5359315
TCTATAC13950.08.5253883
GTCCTAG24600.08.421351
GTTCTAT14950.07.8648991
GTGTAGG25100.07.86322071
CTAGACT20650.07.86027774
ACACCGT11100.07.8193426
CCTACAC24650.07.77630143
TATACGG2704.7207777E-67.77497535
GTATAGG12900.07.7041321
CTTAGAC15100.07.69078353
TAGGACA17800.07.54659754
TAGACAG27950.07.510715
CTAGGAC25450.07.47688253
Produced by FastQC (version 0.11.5)