FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15952736
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACCG5950.08.4686275
TATACTG17700.08.2240855
TACACTG27950.08.162695
GTGTAGG25700.08.0075851
GTCTAGG20850.07.85592171
TAGACCG6250.07.83818535
GTATACG3403.4169716E-87.82333661
CTAGGAC23300.07.80935053
TAGGACA18300.07.7249874
TCTATAC14000.07.59824043
TAGACAG27250.07.55054575
GTATAGA15150.07.4849651
GTCTAGA17950.07.40928841
TGTACTG21350.07.40812065
CGTCAGA25300.07.30416771
CTAGTAC9400.07.2961833
GTATAGG13050.07.29483941
AGGACTG39500.07.2641415
GTCTTGC26100.07.2412011
GTATTAG17450.07.22045231
Produced by FastQC (version 0.11.5)