FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337390
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA423169097.56305059033198No Hit
GCCAAAAA141110.32533389895766807No Hit
CCCAACAA134240.3094948805618125RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA86570.19959007605956577No Hit
GCCACCAA80210.1849268799900401No Hit
GACAACAA77040.1776183372950092No Hit
GCAAACAA73210.16878814217766908No Hit
GCCAACCA60120.13860870246853524No Hit
TCCAACAA51340.11836611418387555No Hit
ACCAACAA49520.11417004235265908No Hit
GCCAACAC49350.1137781015772158No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)