FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4283365
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC244360.5704860547723577No Hit
CTCCTAGTGAACGTAA222250.5188677593434134No Hit
CGACCAGACGCGCTGT211160.492976900170777No Hit
TAGCTTTACCCTATCC196450.45863474161085965No Hit
CCAGGACTGTAACTCG154240.3600907230646933No Hit
ATCTGTACTGGGACTA139270.3251415651012697No Hit
GTTCTCGACCGTAGAG133870.3125346544130608No Hit
CTTCCTTTGGCTCAGC132720.3098498493590903No Hit
CGATCAAACCCGGCTA125350.29264375088277556No Hit
AGACCATTGAATCCAC124680.2910795601122015No Hit
GGGGGGGGGGGGGGGG121830.28442591280453566No Hit
NNNNNNNNNNNNNNNN121470.28358545209198843No Hit
TGCTGCTACTTTCTTG111360.25998251374795284No Hit
ACAGCCGACTTGCGTA102890.24020834087218812No Hit
AACTTACCTCACCTAA93470.21821628556053474No Hit
CGCGTTGGAGCGGTTC89340.20857433349714535No Hit
ATCGAAAGATTTCCGC85850.20042653381161774No Hit
CCCTGAACTAGCTTTG84610.19753161357951052No Hit
GTAACTGTGTGAAAGT81520.1903176591301465No Hit
AGTGGAGTGAGTTGAA79400.1853682793784793No Hit
CAATTCGGATAACGGG78130.1824033207536598No Hit
GGCAACAGAATAGCAA76720.17911151629618302No Hit
GGGTAGAACCAGTCTA74170.1731582529156399No Hit
AAGAACCCTTAACCCA70680.1650104532301123No Hit
TTCCTAAACTAATGCC70540.16468360739745505No Hit
GTGAAGCGATCCCAGG69940.1632828395432096No Hit
TGGAACACTCCTAGTA67780.15824007526792605No Hit
CGCGATCGATATCTGG67310.15714280711543377No Hit
TCCGGGAACGTTCTCC66550.1553685011667229No Hit
TTTAGTCTGAGCAATA66060.15422454075242245No Hit
TGCAGATACTGCCACG62790.14659035594678482No Hit
TGATAGAGAGACTGTT62540.14600670267418256No Hit
CTTTAACTGTCCGTAA62470.1458432797578539No Hit
CACATCCTGAGCCTAT61730.14411566607095125No Hit
AGTCCTGCTCGAACGA60720.1417577068496381No Hit
TGCAGTATGACACCGC59160.13811571042859994No Hit
CTATTGGGACTTGTGA58940.13760209554870995No Hit
AGGTGTGGATCATGTG58650.13692505775249134No Hit
TCTCAGAGATGCATGT58280.13606125090903998No Hit
CATAGGTACAAGGTGC58270.13603790477813588No Hit
ATTTCTGACGGGTAGT57630.13454375240027408No Hit
CTTAGATCTTGTCACA57590.1344503678766577No Hit
TACGTTTACTGCACCG57010.13309629228422049No Hit
GAGTGTTACACCTGCG55860.13041148723025006No Hit
GCAACTTGAAGGTGCA55840.13036479496844186No Hit
TCAAAGCACTCTGCAA55520.12961771877951098No Hit
ATGTACGTGTACTTTC55250.1289873732451005No Hit
TGGCAATTGAGTGGTG54860.128076874139841No Hit
TGAACTGTGAACGTAA54560.12737649021271827No Hit
TCTATGGGATGCTCAA54520.12728310568910192No Hit
TTCTTAGACGTCCCGA53620.12518195390773376No Hit
AGAGTACCTTAATCGC53590.1251119155150215No Hit
CGAGTCTCTGCTACGC52640.12289403307913288No Hit
TGAGGGATGTACGTTG52350.12221699528291426No Hit
GGTCTAAACTCCTACC52350.12221699528291426No Hit
GGGTGTGCTCGCAAAG51260.11967226701436837No Hit
CTACAACGAATCTGTG50450.11778123041113703No Hit
TCCGGGTGAACACCTA49880.11645050094960387No Hit
TCCATTTGAAATCTGC49670.11596023220061798No Hit
CACCAGGTGCGCCACA49260.11500304083355026No Hit
TACTGTTTGCTTAAGC49030.11446607982275618No Hit
GGGATGTGACTGGAAG48950.11427931077552345No Hit
TTGCGGTCTTTTGGGT48370.1129252351830862No Hit
CATTCTATGCCTAGCG48060.11220150512505939No Hit
CTAGCTCACGAATAGT48050.1121781589941553No Hit
GGTCGCATGGTACTAC47770.11152446732884075No Hit
CTCAATTTGGAATCTC47460.11080073727081395No Hit
AGCCAATTGTTATGGC46930.10956339233289716No Hit
TCGGACCGATAGGCGA46750.10914316197662352No Hit
CGAGTCTCTTGGAACG46730.10909646971481535No Hit
AGACTACTGGCCCTAG46680.10897973906029489No Hit
TGCTAGTGAAGGCGTA46300.10809258608593944No Hit
TGTAAGCACGCGCTGT46290.10806923995503535No Hit
GTAATCTCTACACAAA45880.10711204858796763No Hit
CACATTACTGATGGAC45850.10704201019525537No Hit
ACCTCAGCTAATCCGT45490.10620154948270812No Hit
ATGTCCAGACCGCTAG45480.10617820335180402No Hit
AGGCGATGACCTGCGA45420.10603812656637948No Hit
TAGTACCGAACGAGCA45330.10582801138824265No Hit
CTTTCGTCTCGAACGA45270.10568793460281811No Hit
TGCGCAGACATCAGGG44970.10498755067569539No Hit
ACCAAGTTGAAGCTAA44670.10428716674857268No Hit
GGCTGACTGAAACTGA44400.10365682121416223No Hit
CTAACAGTGCATGATC44250.10330662925060088No Hit
ATAGCCAACCGTTCTC44100.10295643728703952No Hit
CATAGTCGAAGATTCC43300.10108874681471226No Hit
AAAGCCTTGAACCAAA42870.10008486318583637No Hit
GGGCCTTGACTATCTT42860.10006151705493227No Hit
TCATCATTGGACTCGC42840.10001482479312411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)