FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139052
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT491937495.72532054550139No Hit
CTTTCCTA949791.8481813377253236No Hit
CTTTACTT151220.29425660608221127No Hit
CTTTCCAT146240.28456610285321104No Hit
CTTTCATT116520.22673442494841461No Hit
CTATCCTT100880.19630079633364286No Hit
ATTTCCTT94660.18419739671830523No Hit
CATTCCTT88130.1714907730063833TruSeq Adapter, Index 27 (100% over 8bp)
CGTTCCTT86790.16888328820179288No Hit
CTTACCTT72810.14167982733002119No Hit
CTTTCCGT70470.1371264583429006No Hit
CTGTCCTT62410.12144263183170748No Hit
CTTTCGTT51710.10062167107863475No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)