FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033672
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT355241.746791026281524No Hit
GAAGCAGTGGAACCTT126180.6204540358523891No Hit
GTTCTTCCTAACGTAA110970.5456632141269585No Hit
CGCTTAAGATAATAGG84380.41491449948664283No Hit
CATGGAGGATCGACAT83460.410390662801081No Hit
TGTCCCATGAGACAAT80600.39632743136553No Hit
GATACGTACCGCTCGA76920.37823208462328245No Hit
AAAGCATGATCTTTGT76320.3752817563500899No Hit
TGAGCGCACAGGACAC74550.3665782879441719No Hit
TAATTGCCTGTCTGGC69700.3427298010691989No Hit
TGTCGAAACTTCGCAA67820.333485439146529No Hit
GGAAGGACTAGGTAAT62510.3073750339287752No Hit
GGGGGGGGGGGGGGGG54580.2683815285847472No Hit
CATGTCAGAGGTTTGT53920.2651361674842354No Hit
AATGATGGAAAGCTAA53270.2619399785216102No Hit
TCGACCTTGTTTCGCC51710.25426912501130955No Hit
GTGAGATCTCGATTAC46200.22717527703582488No Hit
GCTATGATGACCTGCG43090.21188274215311023No Hit
ATGGGTACTTGGCTGC42930.21109598794692555No Hit
AAACGACCTAGGGAGG40230.19781951071755915No Hit
CAGCTCACTGTTTACG38620.18990279651782588No Hit
AGCCTACCTTAACTCG38450.18906687017375468No Hit
TTGCGATGACATTCAG38310.18837846024334307No Hit
TGTTCCTCTTTATGGC37980.18675577969308718No Hit
CTGCCTTGACCATAAC35780.1759379093580479No Hit
CAATGTGACCAACGCC34300.16866043295083966No Hit
CCTGTTGACCCTAGTA34010.16723444095212994No Hit
TAACCAGACATCACAC33690.16566093253976058No Hit
CATGCAAACGAAGGCT33640.1654150718503279No Hit
CATGATGTGTCATGTG33540.16492335047146248No Hit
AGCTTGTGACTCACTC32960.16207136647404302No Hit
ATGGGTACTTTCGGGC32840.1614813008194045No Hit
CACAGCTCTGGACTGA32470.15966193171760246No Hit
CTATTCAACCGAACGA31700.1558756771003387No Hit
ATGCCGCGATGCCACG31270.15376127517121738No Hit
AGAACGCGAGGAATAT30310.14904074993410935No Hit
CATTAGTCTATTCTGG29930.14717220869442071No Hit
TTGTTTGTGATACGCT29920.1471230365565342No Hit
ATTAGCGTGGTCGGTC29660.1458445609714841No Hit
GCAGATACTCGGCTCA28760.1414190685616953No Hit
ATAGGAAACAGACTTC28260.1389604616673682No Hit
TGGGCCATGCGTTCTC28100.13817370746118351No Hit
CACTATACTACGCCAC27990.13763281394443155No Hit
CCGCTATTGTTCGCAA27980.137583641806545No Hit
GCATCTCTGAGCCTAT27900.1371902647034527No Hit
GATACCATGTCTGGAT27730.13635433835938146No Hit
GGGTCGTACCGCTCGA27480.1351250349122179No Hit
CCCAATGCTGGAATGC27380.13463331353335248No Hit
AGCTCGGACCTTAAGC26900.13227305091479843No Hit
TTGTAACCTCATCCTC26810.13183050167381957No Hit
GGGATCAGATGAGTAA26590.13074871464031565No Hit
TGGAGTTACAAGTCTC26590.13074871464031565No Hit
AACGTCCCTTACAGAA26490.13025699326145024No Hit
TCAGGCTCTTAAAGCA26290.1292735505037194No Hit
CTGATTACTGCTTTGA26000.12784755850500965No Hit
GCGTCGATGCTTGAAC25770.1267165993336192No Hit
AACCAACACCGGAATG25710.12642156650629993No Hit
CTGCCATTGCACAGTT25630.1260281894032076No Hit
CGATCAAACCGATCGC25560.1256839844380018No Hit
TTGACGTGACCTCAAA25420.1249955745075902No Hit
GGTAGCCTGTGCCACG25020.12302868899212853No Hit
ATAGCCAACATGGCCC24920.1225369676132631No Hit
CGCTTACTGGCCACGT24790.12189772982073804No Hit
CCTACGGGATGGAACG24680.1213568363039861No Hit
TCCAAAGTGAAGGCCA24680.1213568363039861No Hit
CGCTGAGTGTTAGCTG24580.12086511492512067No Hit
GTAAGAAACGACAAAT24580.12086511492512067No Hit
GGGCTGACTAATACAG24450.12022587713259561No Hit
ATCTTACTGCGCACTG24430.12012753285682252No Hit
TGTTTGTTGCGCTCGA24070.118357335892907No Hit
CTCGGATCTTTCTGAG23690.1164887946532184No Hit
TGGGTGCACTTCTGAG23380.11496445837873562No Hit
CATGTTACTAACGTGG23360.11486611410296253No Hit
CGAGTCTCTTACTTTC23270.11442356486198364No Hit
AGTGGTACTCAACGCC23120.11368598279368552No Hit
TTCTAACACTACGTTG23080.11348929424213935No Hit
GCATAGTACGCTCCTT22970.11294840072538738No Hit
CGCTTCCCTGCGCTGT22770.11196495796765653No Hit
GCGACAAGAAAGGCTG22620.11122737589935841No Hit
TACAACCGATTCCATT22530.11078482665837952No Hit
CTTAGGCCTTATGACC22420.11024393314162755No Hit
TGAGATAGACTCATCC22330.10980138390064868No Hit
GCATATCGAGCCGCTA22320.10975221176276212No Hit
AGAACAATGAGTCGGA22110.10871959686714475No Hit
TACGCCTTGGGTTGAG22010.10822787548827933No Hit
AACCACTGAAAACATG21890.10763780983364082No Hit
TTCACGCTGTGGGCAA21840.1073919491442081No Hit
GAAAGTAACCCTGCGA21770.10704774417900231No Hit
CCTTCAGTGTAGCTTG21740.10690022776534268No Hit
AACAGCTACTCGAGAG21720.1068018834895696No Hit
CCTCCCTTGTTTCGCC21580.10611347355915801No Hit
AGCCGGTACGCGCAAA21580.10611347355915801No Hit
TGGTCCTTGTTTCTGT21430.1053758914908599No Hit
ATTGAGAGATGATCAC21410.10527754721508681No Hit
GGCTTGCGAGTACGTT21400.10522837507720025No Hit
ACCTCCATGGAATTGA21370.10508085866354064No Hit
GTGTACTGAATCACAC21340.104933342249881No Hit
ATCGCAATGTAACTCG21240.10444162087101558No Hit
GTTGCTAGACGCTCGA21030.1034090059753982No Hit
TTCTATGACCAACGCC20890.10272059604498661No Hit
TATCTCCCTGGAATTA20570.10114708763261726No Hit
AGAACATGAAAGCTAA20550.10104874335684418No Hit
AGGTTACTGGTCCCGA20440.1005078498400922No Hit
GGTCAGAGAACCAGTT20370.10016364487488642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)