FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364618
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC327863897.44458360503332Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC178540.5306397338419993No Hit
TGTAGATA160540.4771418330401847No Hit
AGTAGATC61660.1832600313022162No Hit
TGTAGAAC60340.1793368519100831Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC53730.15969123389341672No Hit
TGTACATC53290.15838350742937238No Hit
TCTAGATC33740.10027884294740147ABI Dynabead EcoP Oligo (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)