FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705609
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT401401.083222757716748No Hit
TGCTGCTACCGTTCTC380661.027253549956296No Hit
AAACGACCTCTTCAAG371351.0021294745344154No Hit
ATGGGTTACAATCGAA364620.9839678174356766No Hit
TATGCTTTGGACTGTT362280.977653065933292No Hit
AAAGCGGACTCGGGCT334300.902145909079992No Hit
GCAGGTCTGGCTGTTA333220.8992314083865838No Hit
TCCAAAGTGCTCACGG316590.8543534949316024No Hit
TAGTGCATGAACCCGC296120.7991129123445027No Hit
GAAGTATGAACAGGTG295640.7978175787029878No Hit
TGTGGCTCTTACGATC281690.7601719447464641No Hit
TAACTCACTGGTGTCC274830.7416594681198151No Hit
TTCCCATCTCTTTGTT253150.6831535653113969No Hit
TCACCTGACTCAATGC243440.6569500451882538No Hit
TGAGAATGAGCAGTTT238740.6442665699484215No Hit
GCCTCTTGATCTGTGA232640.6278050382541709No Hit
GGAACTACTACCAAGG230160.6211124811063445No Hit
CTAGAGACTAATCTGC221830.5986330452025564No Hit
GAAGTAATGAGTCTGT221510.5977694894415466No Hit
ATGTCCAGAGTCGGTC219950.5935596551066235No Hit
TCGTATACTGCTAATC218070.5884862650106906No Hit
TCACACAGATTGGGAG213390.5758567620059213No Hit
TTCGTCCGACCTGTGG212960.5746963589520643No Hit
ATTCCTAACTGCTACA211880.571781858258656No Hit
CCATGATGATTCGGGC210530.5681387323918956No Hit
TGCGGTGACCTTAGTG209690.5658718985192448No Hit
AGGTCGAGAAGGGAGG208740.5633082173537467No Hit
TCCGCGAGAGAACAGC205000.5532154093969439No Hit
ACTGATTCTCGGCTCA204270.5512454228171402No Hit
TGGACCTCTAGGGAGG197080.5318424043119498No Hit
GCAATCATGGAACGTG193300.5216416518850209No Hit
TGGCCTGGACCCGAGT181480.48974406096271894No Hit
TTCGATGGAAATCCTG178450.4815672673506568No Hit
AAGGTGCGAACAGATA168330.4542573164087199No Hit
ACGAGGATGGAGCTGA167910.4531238994723944No Hit
TTTCGTAACAGTACGG165360.44624243950184705No Hit
TGGTCCTTGGAGAGGG160930.43428758943536677No Hit
TCCCAGACTGTCTTGA156240.42163110031306594No Hit
TCTCTCTACTCTGGAT154160.41601798786650185No Hit
GCGGTTCGAGTACGTT149970.4047108046207789No Hit
TTCGATGGACAGGTGA138120.37273225534588245No Hit
TGATATCCTCGATGTT137940.3722465052303144No Hit
TCGCCGAGAAGGTAAT137520.3711130882939889No Hit
AGCTGCTCTTGTCACA137280.3704654214732315No Hit
TCCTTCACTTGTCACA134170.3620727389209169No Hit
CTTTCGGTGCTTTGTT131790.3556500429484061No Hit
AACTCCCTGCTTCACC129910.3505766528524731No Hit
TCGGCTTACCTCCTAC121820.3287448837694425No Hit
AGCCCAGTGAAAGTAC121340.3274495501279277No Hit
CCCTTGCCTCCTACGG118150.3188409786353606No Hit
GGGGGGGGGGGGGGGG117140.3161153807646732No Hit
AACCCTCACGCGGTTC116710.3149549777108162No Hit
AAACACGCTGCAGTTT116680.31487401935822157No Hit
ATGGCCGTGATACGCT116390.3140914219498064No Hit
TGTTTGTTGCAAGTTC114350.3085862539733685No Hit
CTAACTGGAACCTTGA109690.29601072320366234No Hit
TACAACGTGAGCAGGT107280.2895070688785568No Hit
CAATGTGTGGTTGACT106890.288454610294826No Hit
AAATAGGACTCTATCT105760.28540517901375995No Hit
TCAAAGGCTCTTAGTG104180.2811413724437738No Hit
TGGCCTTTGCGAAAGC103970.280574663975611No Hit
GGACATTTGGCCGCTA101110.27285663436158536No Hit
ACAGCCGACAAACATG100820.2720740369531702No Hit
CTTTCGGTGAGCCATT99250.26783721650071557No Hit
ACCCTTGTGGGCCATC98980.2671085913273635No Hit
TGAACTGTGTGCTGGC98740.2664609245066061No Hit
AGTGTTCGATTCGCCT93940.2535075880914581No Hit
CAGCCGATGTAATAGG89720.24211944649314052No Hit
GAGGTTGGAGGAATAT89520.2415797241425094No Hit
GATAAGAACATTGGTC83620.22565791479889No Hit
GGCTTGCGAATACGCT81760.22063849693802015No Hit
GTGCTAATGGGAGTCA80460.2171303016589176No Hit
CGACGTTCTTGCTACA79480.21448566214082487No Hit
GTAAAGCCTGAAATCA77400.20887254969426078No Hit
TTGGGAGGAATCTGTG77110.2080899522858456No Hit
TGTACTGGAAATGCTC76220.2056881878255369No Hit
GGGTGAAACCGACTAT72880.19667482456999646No Hit
AGGGACCCTAATCCTG72500.19564935210379725No Hit
GGAACATCTTTACGAC72380.19532551869341852No Hit
AGCTTGTGAACCGTGC69360.18717571119888796No Hit
TACGTGTGAAACGCAG68940.18604229426256252No Hit
TAACGAGCTTACTTTC68800.1856644886171207No Hit
CGGATGAGAACAGGCA68740.18550257191193134No Hit
TCTTGAACTCGTACAT68070.18369450203731694No Hit
TACCACCTGAAACATG68020.18355957144965915No Hit
CATGATGTGCCTAGTA66290.17889097311669958No Hit
ACTTATCTGTCAGGTT65400.1764892086563909No Hit
AACTAGCACTGCCACG60840.16418353906200034No Hit
TGGTAGGGATGTGCTA59290.1600006908446088No Hit
ACGTGTTGATCTTTGT58000.15651948168303778No Hit
AACTACGACATTGCCA56770.15320018922665612No Hit
AACGAACCTGACACCC56730.15309224475652988No Hit
CGTCGTACTTGCGCCA56640.15284936969874588No Hit
TCGGACCGAATGTCCA56200.15166198052735733No Hit
CCTTCAGTGCAACGCC55280.14917925771445395No Hit
GTAGATAGAGCTGAGA54890.14812679913072319No Hit
AGTTTGCGAGCAGGAT54440.14691242384180306No Hit
CTCGGATCTAGTACGG53590.14461860385162062No Hit
GGTAGTTTGTCCAGCG53450.1442407982061788No Hit
TTTAGGGACTGGGCAA53220.14362011750295295No Hit
TACCACCTGTACTTTC53110.1433232702101058No Hit
CTTTAACTGTTCGCCT51510.1390054914050565No Hit
TGAGGAGTGGTCCCAG50900.13735933823563143No Hit
AGAACGCGACACAGTT49530.13366224013380798No Hit
CTTACATTGGAAGGCT48330.13042390603002096No Hit
GAGAAGAGATGCTACA47590.12842693333268565No Hit
CAGGCCATGTCGTAGT46310.1249727102886462No Hit
GAAAGTCCTTATCTGG46190.1246488768782675No Hit
GGGATGTGATGCATGT46050.12427107123282569No Hit
CAAAGACCTGCTTTGA45990.12410915452763635No Hit
CGATCGGTGCGAGGAT45280.12219314018289572No Hit
AATTCTGCTTAGCGAG45210.12200423736017481No Hit
GCTGGACACATTGGTC44820.12095177877644403No Hit
GCCATAATGACCGTTA44730.12070890371866001No Hit
TGCAATGACTCCGTAA44670.12054698701347065No Hit
TCCTCATCTTTCTGGA43720.11798330584797262No Hit
AACTACGACGCTGTTA43560.11755152796746769No Hit
GAAGGACACCCAGCTG43200.1165800277363316No Hit
CCGTTACTGTTGGTTG42840.1156085275051955No Hit
GGACCACTGGCTAGAG40400.1090239148274953No Hit
TGTCTTCCTGAAATCA40370.10894295647490061No Hit
TCGTAGAACCGCACTG40320.10880802588724282No Hit
CCCACCTCTTGACCTA40080.10816035906648543No Hit
GCGTTTAGAAGTCTAC39560.1067570809548444No Hit
ACAAGACCTAAGCTAA39550.10673009483731286No Hit
AGGTGATCTGTTAGTC39440.1064332475444657No Hit
GGCTTAAACTGCATCA39430.10640626142693416No Hit
ATAGCCAACATCTGTG39350.10619037248668169No Hit
ATGGCCGTGGGCCAAA39100.10551571954839271No Hit
TCCGTAAGATTACGCA39030.1053268167256718No Hit
CCTGAGGACTGACTCA39030.1053268167256718No Hit
TATGGGTACCCTTTGC38150.10295203838289468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)