FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6913776
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATC672177597.22292130957092No Hit
CAACCATA391110.5656966612745337No Hit
CCACCATC274500.39703340113998487No Hit
AAACCATC226320.32734644570492305No Hit
CAACCAAC189730.27442312276243835No Hit
CAACCCTC119660.17307474236943748No Hit
CACCCATC116510.16851862137274914No Hit
CAACAATC104500.15114750608061356RNA PCR Primer, Index 36 (100% over 8bp)
CAAACATC97890.141586883925658No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)