FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306183
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC320858097.04786456164102Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC229480.6940934606463103No Hit
TGTAGATA173790.5256514839015263No Hit
AGTAGATC64670.19560320768693082No Hit
TGTAGAAC60620.18335343203930332Illumina DpnII expression Adapter 1 (100% over 8bp)
TGTACATC57050.17255548165361687No Hit
TGAAGATC56740.17161784450527995No Hit
TCTAGATC38140.11535961560506482ABI Dynabead EcoP Oligo (100% over 8bp)
TGTAGATG34270.1036542744306652No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)