FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3279073
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT563031.7170401512866595No Hit
GAAGCAGTGGAACCTT182570.5567732099895306No Hit
GTTCTTCCTAACGTAA167460.5106931135720371No Hit
GATACGTACCGCTCGA152550.4652229456312805No Hit
CGCTTAAGATAATAGG134460.4100549149104031No Hit
CATGGAGGATCGACAT129970.39636202060765346No Hit
TGTCCCATGAGACAAT127790.38971380021121826No Hit
AAAGCATGATCTTTGT123740.3773627485572904No Hit
TGAGCGCACAGGACAC116080.3540024878982566No Hit
TGTCGAAACTTCGCAA107880.32899542035203244No Hit
TAATTGCCTGTCTGGC107280.3271656349218209No Hit
GGGGGGGGGGGGGGGG95340.2907529048606115No Hit
GGAAGGACTAGGTAAT89120.27178412923408535No Hit
AATGATGGAAAGCTAA87730.267545126320762No Hit
CATGTCAGAGGTTTGT86490.2637635697649915No Hit
TCGACCTTGTTTCGCC81830.2495522362570153No Hit
ATGGGTACTTGGCTGC67890.20704022142843417No Hit
GCTATGATGACCTGCG64530.19679342301924962No Hit
AAACGACCTAGGGAGG63050.19227995229139455No Hit
AGCCTACCTTAACTCG62730.19130406672861508No Hit
CAGCTCACTGTTTACG61760.18834591361643976No Hit
TGTTCCTCTTTATGGC60320.1839544285839321No Hit
TTGCGATGACATTCAG58950.17977641851828244No Hit
CTGCCTTGACCATAAC56470.17221330540674148No Hit
CCTGTTGACCCTAGTA55880.1704140164003668No Hit
CAATGTGACCAACGCC55450.1691026701753819No Hit
AGCTTGTGACTCACTC55070.16794380606958123No Hit
TAACCAGACATCACAC54260.1654735957387957No Hit
CACAGCTCTGGACTGA53370.16275941401731525No Hit
CATGCAAACGAAGGCT52180.15913033958072906No Hit
ATGGGTACTTTCGGGC51270.15635516501157493No Hit
ATGCCGCGATGCCACG51180.1560806971970432No Hit
CATGATGTGTCATGTG50790.1548913366674057No Hit
CTATTCAACCGAACGA50750.15476935097205824No Hit
TTGTTTGTGATACGCT50000.15248211918429386No Hit
TGGGCCATGCGTTCTC49070.149645951767466No Hit
GTGAGATCTCGATTAC47960.14626084872157466No Hit
CATTAGTCTATTCTGG47700.14546794170181634No Hit
ATTAGCGTGGTCGGTC47620.14522397031112147No Hit
CCGCTATTGTTCGCAA47100.1436381562716048No Hit
AGAACGCGAGGAATAT46750.14257078143731475No Hit
CACTATACTACGCCAC46210.14092397455012437No Hit
GGGCTGACTAATACAG45900.13997858541118174No Hit
GCATCTCTGAGCCTAT44590.13598355388855327No Hit
AGCTCGGACCTTAAGC44580.1359530574647164No Hit
GGGTCGTACCGCTCGA44520.13577007892169526No Hit
AACCAACACCGGAATG43950.1340317827629943No Hit
GCAGATACTCGGCTCA43840.13369632210078886No Hit
ATAGGAAACAGACTTC43830.13366582567695198No Hit
GGCTTGCGAGTACGTT43630.13305589720021482No Hit
CTGCCATTGCACAGTT43100.1314395867368613No Hit
AACGTCCCTTACAGAA43010.13116511892232957No Hit
TTGACGTGACCTCAAA42400.1293048370682812No Hit
GGTAGCCTGTGCCACG42110.12842044077701228No Hit
TTGTAACCTCATCCTC42100.12838994435317544No Hit
CCCAATGCTGGAATGC42090.12835944792933857No Hit
CTGATTACTGCTTTGA41770.1273835623665591No Hit
TGGAGTTACAAGTCTC41360.12613320898924787No Hit
ATAGCCAACATGGCCC40600.12381548077764662No Hit
CCTACGGGATGGAACG40410.12323604872474629No Hit
CGCTTACTGGCCACGT39870.12158924183755591No Hit
CGATCAAACCGATCGC39860.12155874541371907No Hit
TGTTTGTTGCGCTCGA39300.11985094567885496No Hit
CGCTGAGTGTTAGCTG39130.11933250647362836No Hit
ATCTTACTGCGCACTG39000.1189360529637492No Hit
GATACCATGTCTGGAT38440.11722825322888512No Hit
CGCTTCCCTGCGCTGT38390.11707577110970083No Hit
TCCAAAGTGAAGGCCA38250.1166488211759848No Hit
GGGATCAGATGAGTAA38230.11658782832831109No Hit
CATGTTACTAACGTGG38150.1163438569376162No Hit
TCAGGCTCTTAAAGCA37740.11509350356030501No Hit
CTCGGATCTTTCTGAG37320.11381265375915693No Hit
TGGGTGCACTTCTGAG37210.11347719309695149No Hit
TACAACCGATTCCATT37130.1132332217062566No Hit
TTCTAACACTACGTTG36410.11103747919000279No Hit
AGCCGGTACGCGCAAA36270.11061052925628675No Hit
CTTAGGCCTTATGACC36110.11012258647489703No Hit
AGTGGTACTCAACGCC36050.10993960793187586No Hit
CCTCCCTTGTTTCGCC35820.10923819018362811No Hit
ACCTGCTTGCCAAACT35540.10838429031619608No Hit
CCTTCAGTGTAGCTTG35410.10798783680631692No Hit
CGAGTCTCTTACTTTC35200.10734741190574287No Hit
GTAAGAAACGACAAAT35110.10707294409121114No Hit
AACAGCTACTCGAGAG34940.10655450488598454No Hit
GCATAGTACGCTCCTT34790.10609705852843167No Hit
TACGCCTTGGGTTGAG34650.10567010859471565No Hit
AACCACTGAAAACATG34550.10536514435634706No Hit
TGAGATAGACTCATCC34530.10530415150867332No Hit
AGAACAATGAGTCGGA34450.10506018011797846No Hit
GTCCTGACTTTACGAC34440.10502968369414162No Hit
ATTGAGAGATGATCAC34360.10478571230344673No Hit
CATGTCAGAACAGGTG34100.1039928052836884No Hit
GTGTACTGAATCACAC33990.10365734462148296No Hit
ATCGCAATGTAACTCG33860.1032608911116038No Hit
TGGTCCTTGTTTCTGT33780.10301691972090891No Hit
TATCTCCCTGGAATTA33780.10301691972090891No Hit
TTCACGCTGTGGGCAA33560.10234599839649802No Hit
GCATGGGCTATGTGGG33120.10100415574767624No Hit
ACCTCCATGGAATTGA33120.10100415574767624No Hit
TTCTATGACCAACGCC32970.10054670939012338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)