FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17181572
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA17100.010.15422823
ACGAGAC17800.09.83262922
CTTATAC22000.09.7384571
TACACCG7900.09.2141925
TAGACTG22800.09.0253385
TATACAC34650.08.9285873
CCCACGA19550.08.73598319
TCTCCGA21500.08.59424411
TAGTACG3951.2732926E-118.5054084
AGGACCG19350.08.4642015
TACACTG31450.08.4124445
GTATAGG15200.08.383381
TAGGACA21750.08.3025494
ATCTCCG21000.08.26560410
TAGACAG30150.08.2644455
GGACCGT10900.08.2193426
CTAGGAC26400.08.1129943
TGGACCG11950.08.0828075
CACGAGA22050.07.936688421
CGATACT2653.6064303E-67.9236714
Produced by FastQC (version 0.11.5)