FastQCFastQC Report
Wed 8 May 2024
EGAF00004949155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4090311
Total Bases32.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC401687498.20461084743923Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC215600.5270992841375631No Hit
TGTAGATA133830.32718783486145675No Hit
TGAAGATC65200.15940108221599775No Hit
AGTAGATC53310.13033238792844848No Hit
TGTAGAAC46750.11429448763186954Illumina DpnII expression Adapter 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)