FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076436
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG199805396.22511842406894No Hit
GGACAGGA241881.164880593478441No Hit
GGCCAGGG220491.0618675461222982ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGC30960.14910163376092497No Hit
GGGCAGGG30790.1482829232396279No Hit
GGAAAGGG26630.12824859518906434No Hit
GGTCAGGG22320.10749187550206218No Hit
GGACACGG22110.10648052721104816No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)