FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7731263
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA751413197.19150674346481No Hit
GTGGCGCA661310.8553712375325997No Hit
GTGGAGCC167270.21635533547364771No Hit
GGGGAGCA136010.17592209707521267No Hit
GAGGAGCA120070.1553045084612954No Hit
GTGGAGCT105160.13601917306396122No Hit
GTGGACCA100270.12969420390950354ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGGAGAA89120.11527223947756014No Hit
GTGCAGCA86100.11136602130854946No Hit
GTGTAGCA79420.10272577714663181No Hit
CTGGAGCA77690.10048810912266211No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)