FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980168
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC389568797.87745140406133Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC271920.6831872423475592No Hit
TGTAGATA136730.34352821287945634No Hit
TGAAGATC69760.17526898362079188No Hit
AGTAGATC55070.13836099380729658No Hit
TGTAGAAC46450.1167036165307595Illumina DpnII expression Adapter 1 (100% over 8bp)
TGTACATC42370.10645279294743337No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)