FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3621325
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT346912095.79698038701304No Hit
CTTTCCTA658061.8171801757643955No Hit
CTTTCCAT102930.2842329810221397No Hit
CTTTACTT93740.25885552939876977No Hit
CTTTCATT88050.24314304846982804No Hit
CATTCCTT71890.19851849806355407TruSeq Adapter, Index 27 (100% over 8bp)
ATTTCCTT67580.18661677700841545No Hit
CTATCCTT67280.185788350948893No Hit
CTTACCTT50670.13992116145333547No Hit
CGTTCCTT49730.13732542646683188No Hit
CTTTCCGT45710.12622451726923156No Hit
CTGTCCTT42850.11832685550178457No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)