FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018001
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC292072996.77693943772717Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC261020.8648771156802135No Hit
TGTAGATA159450.528329844821125No Hit
AGTAGATC60250.1996354540637992No Hit
TGAAGATC58310.19320735811552084No Hit
TGTAGAAC54170.17948966882383405Illumina DpnII expression Adapter 1 (100% over 8bp)
TGTACATC49580.16428092634826827No Hit
TCTAGATC36440.12074217337900153ABI Dynabead EcoP Oligo (100% over 8bp)
TTTAGATC34110.11302183133802807No Hit
TATAGATC34030.11275675521644957No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)