FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5114466
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT488717495.55589967750299No Hit
CTTTCCTA946601.8508286104551286No Hit
CTTTACTT156800.30658137134942337No Hit
CTTTCCAT147290.28798705475801384No Hit
CTTTCATT126740.24780690691853266No Hit
CTATCCTT107850.21087245471961297No Hit
ATTTCCTT104640.2045961396556356No Hit
CATTCCTT100070.19566070045240305TruSeq Adapter, Index 27 (100% over 8bp)
CGTTCCTT85190.16656675398761084No Hit
CTTTCCGT84830.1658628681860433No Hit
CTTACCTT71700.14019058881220445No Hit
CTTTCGTT62640.12247612947275434No Hit
CTGTCCTT54930.10740124188918256No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)