FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687994
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG360529397.75756142770298No Hit
TAATGGTG89310.24216416837988347No Hit
TATTGGTA82230.22296674018450138No Hit
AATTGGTG66440.18015213690694723No Hit
TATTGGGG60800.16485927037842252No Hit
TATTGGTC55710.15105772948654472No Hit
TATTGCTG52150.14140478536570286No Hit
TATTGGAG52100.14126921030782588No Hit
TATTCGTG46180.12521712345519No Hit
TATAGGTG45260.12272254239025335RNA PCR Primer, Index 39 (100% over 8bp)
TATTGGTT43660.11838414053818959No Hit
TTTTGGTG38690.10490797978521657RNA PCR Primer, Index 28 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)