FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004949243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17994877
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1845591.025619680534632No Hit
TGCTGCTACCGTTCTC1834271.019329001248522No Hit
AAACGACCTCTTCAAG1801731.0012460768695446No Hit
ATGGGTTACAATCGAA1746650.9706373652901322No Hit
TATGCTTTGGACTGTT1722690.9573224646103443No Hit
AAAGCGGACTCGGGCT1604120.8914314890843655No Hit
GCCTCTTGATCTGTGA1525340.8476523623918073No Hit
GCAGGTCTGGCTGTTA1512040.8402613699443457No Hit
TCCAAAGTGCTCACGG1507970.8379996151126791No Hit
TAGTGCATGAACCCGC1433440.7965822717210015No Hit
TGTGGCTCTTACGATC1345100.7474905218857567No Hit
TAACTCACTGGTGTCC1341240.7453454669348393No Hit
GAAGTATGAACAGGTG1305220.7253286588177291No Hit
TTCCCATCTCTTTGTT1228090.6824664597596305No Hit
TCACCTGACTCAATGC1175030.6529802898902838No Hit
TGAGAATGAGCAGTTT1125080.6252223896834638No Hit
GGAACTACTACCAAGG1101200.6119519461011043No Hit
ATGTCCAGAGTCGGTC1072960.5962585907089001No Hit
GAGCCTGACCGAGTTT1063280.5908792819200709No Hit
CTAGAGACTAATCTGC1055510.5865613863323434No Hit
TCGTATACTGCTAATC1053700.5855555445030272No Hit
TTCGTCCGACCTGTGG1051430.584294074363498No Hit
TGCGGTGACCTTAGTG1022160.568028333841904No Hit
TCACACAGATTGGGAG1018860.566194478572985No Hit
GAAGTAATGAGTCTGT1015730.5644550946361011No Hit
ATTCCTAACTGCTACA1012590.5627101535620388No Hit
TCCGCGAGAGAACAGC996870.5539743339173699No Hit
AGGTCGAGAAGGGAGG988580.5493674671963582No Hit
ACTGATTCTCGGCTCA975350.5420153747091464No Hit
TGGACCTCTAGGGAGG953380.5298063443278884No Hit
TGGCCTGGACCCGAGT947550.5265665333527981No Hit
CCATGATGATTCGGGC923950.5134516896114377No Hit
GCAATCATGGAACGTG843480.46873340673570596No Hit
TTCGATGGAAATCCTG835100.46407652578008735No Hit
ACGAGGATGGAGCTGA821210.45635766223909174No Hit
AAGGTGCGAACAGATA797260.44304831869648237No Hit
TTTCGTAACAGTACGG792170.4402197358726042No Hit
TCCCAGACTGTCTTGA775490.43095043105879527No Hit
TGGTCCTTGGAGAGGG762030.42347052441647703No Hit
TCTCTCTACTCTGGAT747540.41541823264476885No Hit
TCGCCGAGAAGGTAAT667430.3709000067074646No Hit
TTCGATGGACAGGTGA664810.3694440367666864No Hit
TGATATCCTCGATGTT661400.3675490529888034No Hit
AGCTGCTCTTGTCACA651630.36211972996536734No Hit
TCCTTCACTTGTCACA649390.36087493123737385No Hit
AGCCCAGTGAAAGTAC638730.3549510230050475No Hit
AACTCCCTGCTTCACC637750.35440642356155033No Hit
GCGGTTCGAGTACGTT630950.3506275702801414No Hit
CTTTCGGTGCTTTGTT629270.34969397123414625No Hit
CCCTTGCCTCCTACGG601060.3340172872534778No Hit
AACCCTCACGCGGTTC596040.3312276043898494No Hit
ATGGCCGTGATACGCT592640.3293381777491449No Hit
GGGGGGGGGGGGGGGG585220.3252147819626664No Hit
TCGGCTTACCTCCTAC580380.3225251275682518No Hit
TGTTTGTTGCAAGTTC552620.30709851476061767No Hit
AAACACGCTGCAGTTT549580.30540914505834077No Hit
ACCCTTGTGGGCCATC539870.30001316485797597No Hit
TGGCCTTTGCGAAAGC525710.29214425861315974No Hit
CTAACTGGAACCTTGA515020.28620367896929777No Hit
TACAACGTGAGCAGGT512530.28481995181184067No Hit
CAATGTGTGGTTGACT508650.28266378258656616No Hit
AAATAGGACTCTATCT506380.281402312447037No Hit
TCAAAGGCTCTTAGTG485100.2695767245310985No Hit
CTTTCGGTGAGCCATT480170.266837055902077No Hit
ACAGCCGACAAACATG474530.26370283053337906No Hit
TGAACTGTGTGCTGGC469050.2606575193595377No Hit
AGTGTTCGATTCGCCT448850.2494321022588818No Hit
CAGCCGATGTAATAGG439380.24416949335080199No Hit
GGACATTTGGCCGCTA434860.2416576673461008No Hit
GAGGTTGGAGGAATAT399770.222157672986595No Hit
CGACGTTCTTGCTACA379470.21087668451415367No Hit
TTGGGAGGAATCTGTG372060.20675884586485366No Hit
TGTACTGGAAATGCTC362830.2016296082490589No Hit
GTGCTAATGGGAGTCA361530.20090718041584835No Hit
AGGGACCCTAATCCTG348250.19352730224274386No Hit
GGAACATCTTTACGAC347340.1930216027594965No Hit
GGGTGAAACCGACTAT333410.18528051066978674No Hit
TAACGAGCTTACTTTC332560.18480815400961062No Hit
GGACCACTGGCTAGAG331700.18433024021225597No Hit
CGGATGAGAACAGGCA331010.18394679774693654No Hit
TCTTGAACTCGTACAT330570.18370228371108066No Hit
TACCACCTGAAACATG329620.1831743556791191No Hit
TACGTGTGAAACGCAG327020.18172950001269805No Hit
AGCTTGTGAACCGTGC319810.1777228041069689No Hit
CATGATGTGCCTAGTA312250.17352160839999073No Hit
ACTTATCTGTCAGGTT308990.17170998167978588No Hit
ACGTGTTGATCTTTGT292200.16237954835701293No Hit
AACTAGCACTGCCACG284750.15823948115899875No Hit
TGGTAGGGATGTGCTA280390.1558165693491542No Hit
AACGAACCTGACACCC277470.15419388529301978No Hit
TCGGACCGAATGTCCA275100.15287684378170519No Hit
CGTCGTACTTGCGCCA272930.15167094501396147No Hit
GTCCGAGTGGTCTTGA272000.15115413125635702No Hit
GTAGATAGAGCTGAGA271470.15085960298589426No Hit
AACTACGACATTGCCA271320.15077624592821612No Hit
CTCGGATCTAGTACGG271140.15067621745900237No Hit
TACCACCTGTACTTTC261280.14519688020095942No Hit
TTTAGGGACTGGGCAA261270.14519132306378088No Hit
CCTTCAGTGCAACGCC260810.14493569475356793No Hit
AGTTTGCGAGCAGGAT260150.1445689236997841No Hit
GGTAGTTTGTCCAGCG258930.14389095296400192No Hit
TGAGGAGTGGTCCCAG254530.1414458126054432No Hit
GGCTTGCGAATACGCT247920.1377725449304266No Hit
CTTTAACTGTTCGCCT244010.13559970429361645No Hit
GCCATAATGACCGTTA241980.13447160544637232No Hit
AGAACGCGACACAGTT236420.13138183717510266No Hit
GTCCGTTACGGATGGT229620.12760298389369373No Hit
CTTACATTGGAAGGCT227110.12620814246187956No Hit
CGATCGGTGCGAGGAT226210.12570800011581074No Hit
CCGTTACTGTTGGTTG224010.12448542993653139No Hit
CAAAGACCTGCTTTGA223960.12445764425063867No Hit
GGGATGTGATGCATGT223150.12400751613917671No Hit
TGCAATGACTCCGTAA220420.12249041768943461No Hit
CAGGCCATGTCGTAGT212560.11812250786710017No Hit
GATAAGAACATTGGTC211510.1175390084633532No Hit
GAACCATACTTTGGGT210780.11713333744931961No Hit
AATTCTGCTTAGCGAG210310.1168721520019281No Hit
TCCTCATCTTTCTGGA210000.11669988074939329No Hit
GAGAAGAGATGCTACA209600.11647759526225158No Hit
AACTACGACGCTGTTA209330.11632755255843094No Hit
GAAAGTCCTTATCTGG205430.11416026905879935No Hit
CCCACCTCTTGACCTA204620.1137101409473374No Hit
TGTCTTCCTGAAATCA199620.11093157235806614No Hit
ATGGCCGTGGGCCAAA197590.10980347351082201No Hit
TCGTAGAACCGCACTG195490.10863647470332806No Hit
CCTGAGGACTGACTCA194710.10820301800340175No Hit
TCCGTAAGATTACGCA190870.10606907732684141No Hit
TATGGGTACCCTTTGC188440.10471869299245558No Hit
GAAGGACACCCAGCTG187450.10416853641177987No Hit
AGGTGATCTGTTAGTC187010.10392402237592399No Hit
ACAAGACCTAAGCTAA185870.10329050873757015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)