FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069685
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT333521.086495845664946No Hit
TGCTGCTACCGTTCTC317371.0338845842488724No Hit
AAACGACCTCTTCAAG305270.9944668589773868No Hit
TATGCTTTGGACTGTT296550.9660600354759528No Hit
ATGGGTTACAATCGAA295040.961140963975131No Hit
GCAGGTCTGGCTGTTA273580.8912315107250417No Hit
AAAGCGGACTCGGGCT271190.883445695568112No Hit
TCCAAAGTGCTCACGG257630.8392717819580836No Hit
TAGTGCATGAACCCGC245970.8012874285146523No Hit
GAAGTATGAACAGGTG243780.794153146006838No Hit
TAACTCACTGGTGTCC230210.7499466557643537No Hit
TGTGGCTCTTACGATC230060.7494580062775171No Hit
GCCTCTTGATCTGTGA219300.7144055497551052No Hit
TTCCCATCTCTTTGTT207490.675932546824837No Hit
TCACCTGACTCAATGC195320.6362867851261612No Hit
GGAACTACTACCAAGG192870.6283055101744968No Hit
TGAGAATGAGCAGTTT189640.617783257891282No Hit
GAAGTAATGAGTCTGT186080.6061859767370268No Hit
CTAGAGACTAATCTGC182380.5941326227283907No Hit
ATGTCCAGAGTCGGTC181990.5928621340626156No Hit
TCACACAGATTGGGAG179550.5849134357434069No Hit
TTCGTCCGACCTGTGG177930.5796360212855716No Hit
TCGTATACTGCTAATC177460.5781049195601503No Hit
TGCGGTGACCTTAGTG176730.5757268253908788No Hit
CCATGATGATTCGGGC171650.5591778961033461No Hit
TCCGCGAGAGAACAGC170810.5564414589770612No Hit
ATTCCTAACTGCTACA168270.5481669943332947No Hit
ACTGATTCTCGGCTCA166540.5425312369184461No Hit
AGGTCGAGAAGGGAGG165710.5398273764246169No Hit
TGGACCTCTAGGGAGG164410.5355924142053664No Hit
GCAATCATGGAACGTG161440.5259171543660017No Hit
TGGCCTGGACCCGAGT156110.5085538092670747No Hit
TTCGATGGAAATCCTG142070.4628162172991691No Hit
ACGAGGATGGAGCTGA140430.45747364957642234No Hit
AAGGTGCGAACAGATA133860.4360708020529794No Hit
TTTCGTAACAGTACGG133840.43600564878806786No Hit
TCCCAGACTGTCTTGA131280.4276660308793899No Hit
GCGGTTCGAGTACGTT130190.42411517794171066No Hit
TGGTCCTTGGAGAGGG129730.42261665284874506No Hit
TCTCTCTACTCTGGAT127260.4145702246321691No Hit
TCCTTCACTTGTCACA112630.366910611349373No Hit
AGCTGCTCTTGTCACA111800.36420675085554377No Hit
TCGCCGAGAAGGTAAT111180.3621869996432859No Hit
TTCGATGGACAGGTGA111070.36182865668627234No Hit
TGATATCCTCGATGTT109530.3568118552880833No Hit
AACTCCCTGCTTCACC108490.3534238855126829No Hit
CTTTCGGTGCTTTGTT106460.34681082912416095No Hit
AGCCCAGTGAAAGTAC104540.3405561156926525No Hit
ATGGCCGTGATACGCT99190.32312761732881384No Hit
AACCCTCACGCGGTTC99120.32289958090162346No Hit
CCCTTGCCTCCTACGG98580.3211404427490117No Hit
TCGGCTTACCTCCTAC98370.32045633346744046No Hit
TGTTTGTTGCAAGTTC94410.3075559870149543No Hit
AAACACGCTGCAGTTT92220.30042170450713995No Hit
CTAACTGGAACCTTGA90100.29351545842651605No Hit
GGGGGGGGGGGGGGGG88290.2876190879520211No Hit
TGGCCTTTGCGAAAGC87430.28481749756082464No Hit
TACAACGTGAGCAGGT86020.28022419238456064No Hit
CAATGTGTGGTTGACT85860.27970296626526825No Hit
TCAAAGGCTCTTAGTG84740.27605438343022165No Hit
ACCCTTGTGGGCCATC84500.2752725442512831No Hit
ACAGCCGACAAACATG83980.2735785593635829No Hit
AAATAGGACTCTATCT83650.2725035304925424No Hit
GGACATTTGGCCGCTA82700.26940875040924395No Hit
CTTTCGGTGAGCCATT82060.2673238459320745No Hit
TGAACTGTGTGCTGGC78060.2542931929497652No Hit
GAGGTTGGAGGAATAT75920.24732179360422976No Hit
AGTGTTCGATTCGCCT75770.24683314411739316No Hit
CAGCCGATGTAATAGG74090.24136026986482326No Hit
GATAAGAACATTGGTC73490.23940567191747686No Hit
GGCTTGCGAATACGCT70880.2309031708465201No Hit
GTGCTAATGGGAGTCA67870.2210976044773324No Hit
GTAAAGCCTGAAATCA65850.2145171247212662No Hit
CGACGTTCTTGCTACA64680.21070565872394073No Hit
TGTACTGGAAATGCTC63110.20559112742838437No Hit
TTGGGAGGAATCTGTG63030.20533051436873814No Hit
GGAACATCTTTACGAC59970.1953620648372716No Hit
GGGTGAAACCGACTAT59890.1951014517776254No Hit
AGGGACCCTAATCCTG58770.1914528689425788No Hit
TCTTGAACTCGTACAT57020.1857519582628185No Hit
TAACGAGCTTACTTTC56750.18487238918651264No Hit
AGCTTGTGAACCGTGC56200.1830806744014451No Hit
CGGATGAGAACAGGCA56140.18288521460671045No Hit
TACGTGTGAAACGCAG55440.18060485033480633No Hit
TACCACCTGAAACATG55430.18057227370235057No Hit
ACTTATCTGTCAGGTT53500.17428498363838635No Hit
CATGATGTGCCTAGTA53130.17307964823752273No Hit
AACTAGCACTGCCACG51870.16897499254809534No Hit
ACGTGTTGATCTTTGT49080.15988611209293463No Hit
TCGGACCGAATGTCCA48210.15705194506928236No Hit
TGGTAGGGATGTGCTA47930.1561397993605207No Hit
AACGAACCTGACACCC47350.15425035467808587No Hit
AACTACGACATTGCCA46870.15268667632020874No Hit
GTAGATAGAGCTGAGA46750.15229575673073947No Hit
CTCGGATCTAGTACGG46670.1520351436710933No Hit
GGTAGTTTGTCCAGCG45910.14955931960445454No Hit
CGTCGTACTTGCGCCA45900.14952674297199875No Hit
TTTAGGGACTGGGCAA45800.14920097664744103No Hit
CCTTCAGTGCAACGCC45500.14822367767376785No Hit
AGTTTGCGAGCAGGAT45380.14783275808429855No Hit
TACCACCTGTACTTTC43950.143174299643123No Hit
TGAGGAGTGGTCCCAG43600.14203411750717093No Hit
CTTTAACTGTTCGCCT42870.1396560233378995No Hit
CAGGCCATGTCGTAGT41920.13656124325460106No Hit
GCCATAATGACCGTTA41440.13499756489672393No Hit
GGGATGTGATGCATGT40580.13219597450552745No Hit
GGACCACTGGCTAGAG40420.13167474838623505No Hit
AGAACGCGACACAGTT40390.13157701848886774No Hit
CTTACATTGGAAGGCT40170.13086033257484073No Hit
GAAAGTCCTTATCTGG40150.13079517930992918No Hit
GAGAAGAGATGCTACA39570.12890573462749436No Hit
CGATCGGTGCGAGGAT39490.12864512156784816No Hit
GAAGGACACCCAGCTG38650.12590868444156322No Hit
TGCAATGACTCCGTAA38390.1250616919977131No Hit
GCTGGACACATTGGTC38280.1247033490406996No Hit
CAAAGACCTGCTTTGA37120.12092445967582995No Hit
AACTACGACGCTGTTA37010.12056611671881641No Hit
AATTCTGCTTAGCGAG36500.118904708463572No Hit
GCGTTTAGAAGTCTAC35930.11704784041359294No Hit
CCGTTACTGTTGGTTG35720.1163637311320217No Hit
GGCTTAAACTGCATCA35660.11616827133728706No Hit
TCCTCATCTTTCTGGA35370.11522354899606962No Hit
TCGTAGAACCGCACTG33870.11033705412770367No Hit
TGTCTTCCTGAAATCA33770.11001128780314592No Hit
CCCACCTCTTGACCTA33760.10997871117069016No Hit
TCCGTAAGATTACGCA33410.1088385290347381No Hit
ATAGCCAACATCTGTG33200.10815441975316686No Hit
ATGGCCGTGGGCCAAA33030.10760061700141872No Hit
CCTGAGGACTGACTCA32830.10694908435230324No Hit
AGGTGATCTGTTAGTC32370.10545055925933768No Hit
GCGTTAATGGTACGTT32050.10440810702075293No Hit
TATGGGTACCCTTTGC31820.10365884447427016No Hit
ACAAGACCTAAGCTAA31390.10225804927867191No Hit
ATCTCTTGACGGTACC30960.10085725408307367No Hit
GGCTTCTACATCAGCT30750.10017314480150244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)