FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17994877
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCC195800.10880874595586287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC29650.019.45773922
TCTCCGA31600.018.78157611
TCCGAGC32100.018.66350413
CCCACGA32250.018.05806419
ATCTCCG32950.017.92710910
CCACGAG33050.017.62139520
CGAGCCC34900.017.28646915
CACGAGA33750.017.13304521
CCGAGCC36850.016.33373314
CTCCGAG37200.016.21763612
GCCCACG36850.015.99350618
CTTATAC44850.014.577281
GACTAAG41150.014.36357726
ACTAAGC43400.013.81267127
CTAAGCC44000.013.40204828
GAGACTA46550.012.69656624
TATACAC70500.012.5682943
AGCGTCA10900.012.1999713
ACATCTC58550.011.666658
CGAGACT50500.011.53626423
Produced by FastQC (version 0.11.5)