FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144535
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT553261.7594334297439844No Hit
GAAGCAGTGGAACCTT196460.6247664599058367No Hit
GTTCTTCCTAACGTAA162140.5156247267084004No Hit
GATACGTACCGCTCGA142410.4528809506015993No Hit
CGCTTAAGATAATAGG128670.40918609587745086No Hit
TGTCCCATGAGACAAT126730.4030166622409991No Hit
CATGGAGGATCGACAT124860.3970698370347285No Hit
AAAGCATGATCTTTGT116480.3704204278216016No Hit
TGAGCGCACAGGACAC111780.3554738617951462No Hit
TGTCGAAACTTCGCAA106680.3392552475962265No Hit
GGGGGGGGGGGGGGGG105000.3339126452718764No Hit
TAATTGCCTGTCTGGC101630.3231956394188648No Hit
GGAAGGACTAGGTAAT94320.2999489590670799No Hit
AATGATGGAAAGCTAA83300.2649040319156886No Hit
CATGTCAGAGGTTTGT81350.2587027970749252No Hit
TCGACCTTGTTTCGCC77410.24617312257615198No Hit
GTGAGATCTCGATTAC68090.21653440015773398No Hit
ATGGGTACTTGGCTGC62970.20025218355019103No Hit
AAACGACCTAGGGAGG61600.19589541855950082No Hit
AGCCTACCTTAACTCG60360.19195206922486155No Hit
TTGCGATGACATTCAG59330.188676545180766No Hit
CAGCTCACTGTTTACG58610.1863868584703303No Hit
GCTATGATGACCTGCG57090.18155307541496596No Hit
TGTTCCTCTTTATGGC54290.17264873820771592No Hit
CAATGTGACCAACGCC53380.16975482861535965No Hit
CTGCCTTGACCATAAC53210.16921420814206234No Hit
CCTGTTGACCCTAGTA52950.16838737682996055No Hit
CATGCAAACGAAGGCT51660.16428502147376323No Hit
TAACCAGACATCACAC51010.16221794319350874No Hit
AGCTTGTGACTCACTC50540.1607232865908632No Hit
CATGATGTGTCATGTG50000.1590060215580364No Hit
CACAGCTCTGGACTGA49710.1580837866329998No Hit
CTATTCAACCGAACGA49450.157256955320898No Hit
ATGCCGCGATGCCACG49200.1564619252131078No Hit
ATGGGTACTTTCGGGC48360.1537906240509328No Hit
TGGGCCATGCGTTCTC47510.1510875216844462No Hit
AGAACGCGAGGAATAT46940.14927485303868457No Hit
TTGTTTGTGATACGCT46670.14841622052227116No Hit
ATTAGCGTGGTCGGTC46610.14822541329640154No Hit
CATTAGTCTATTCTGG45750.14549050972560332No Hit
CCGCTATTGTTCGCAA45240.14386864830571133No Hit
GGGCTGACTAATACAG44490.1414835579823408No Hit
GGGTCGTACCGCTCGA44350.1410383411219783No Hit
CACTATACTACGCCAC43330.13779461828219436No Hit
AACCAACACCGGAATG43030.1368405821528461No Hit
AGCTCGGACCTTAAGC42200.13420108219498272No Hit
TTGACGTGACCTCAAA41980.13350145570012736No Hit
GGTAGCCTGTGCCACG41320.13140257621556128No Hit
AACGTCCCTTACAGAA41230.13111636537675683No Hit
ATAGGAAACAGACTTC41090.13067114851639433No Hit
CCCAATGCTGGAATGC40830.12984431720429254No Hit
CTGCCATTGCACAGTT39930.12698220881624786No Hit
CCTACGGGATGGAACG39440.12542394980497912No Hit
TTGTAACCTCATCCTC39280.1249151305359934No Hit
ATAGCCAACATGGCCC39280.1249151305359934No Hit
TGGAGTTACAAGTCTC39010.12405649801957999No Hit
GGCTTGCGAGTACGTT38730.123166064298855No Hit
CGCTTACTGGCCACGT38520.12249823900831124No Hit
CTGATTACTGCTTTGA38290.12176681130914428No Hit
ATCTTACTGCGCACTG37920.12059016674961481No Hit
GCAGATACTCGGCTCA37370.1188411005124764No Hit
CGCTGAGTGTTAGCTG37140.11810967281330943No Hit
TGTTTGTTGCGCTCGA37020.11772805836157015No Hit
TGGGTGCACTTCTGAG36930.1174418475227657No Hit
CTCGGATCTTTCTGAG36900.11734644390983086No Hit
CATGTTACTAACGTGG36750.11686942584515675No Hit
CGATCAAACCGATCGC36750.11686942584515675No Hit
TCAGGCTCTTAAAGCA36690.11667861861928712No Hit
GCGTCGATGCTTGAAC36250.11527936562957639No Hit
CGCTTCCCTGCGCTGT36190.11508855840370676No Hit
CGAGTCTCTTACTTTC36030.11457973913472103No Hit
TCCAAAGTGAAGGCCA35970.11438893190885138No Hit
AGCCGGTACGCGCAAA35770.11375290782261924No Hit
TTCTAACACTACGTTG35370.11248085965015495No Hit
GTAAGAAACGACAAAT35140.11174943195098798No Hit
GATACCATGTCTGGAT35120.11168582954236476No Hit
CTTAGGCCTTATGACC34790.11063638980008174No Hit
CCTTCAGTGTAGCTTG34720.11041378136990047No Hit
AGTGGTACTCAACGCC34570.10993676330522638No Hit
TACAACCGATTCCATT33920.1078696850249719No Hit
GCGACGTTGCTTAGTG33820.10755167298185583No Hit
TACGCCTTGGGTTGAG33680.10710645612149333No Hit
ACCTGCTTGCCAAACT33610.10688384769131207No Hit
CCTCCCTTGTTTCGCC33510.106565835648196No Hit
GGTCAGAGAACCAGTT33430.10631142601370315No Hit
ATTGAGAGATGATCAC33350.10605701637921028No Hit
TATCTCCCTGGAATTA33300.10589801035765226No Hit
AGAACAATGAGTCGGA33260.10577080554040581No Hit
GTCCTGACTTTACGAC33210.10561179951884778No Hit
TTCACGCTGTGGGCAA33190.10554819711022458No Hit
AACAGCTACTCGAGAG33180.10551639590591297No Hit
ATCGCAATGTAACTCG32820.10437155255069508No Hit
AACCACTGAAAACATG32660.10386273328170939No Hit
GAAAGTAACCCTGCGA32480.10329031160410045No Hit
TGAGATAGACTCATCC32380.10297229956098437No Hit
AGAACATGAAAGCTAA31920.10150944416265045No Hit
CATGTCAGAACAGGTG31800.10112782971091117No Hit
TGGTCCTTGTTTCTGT31610.10052360682899061No Hit
AAAGGATACTGCTGGC31540.10030099839880938No Hit
AGGTTACTGGTCCCGA31460.1000465887643165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)