FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269548
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC319906897.84435035056833Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA215550.6592654397488583No Hit
ATCAGATC67320.20590002043095867Illumina PCR Primer Index 9 (100% over 8bp)
ATATGATC53660.16412054510287047No Hit
ATCTGCTC48000.14680928372973878No Hit
ATCTGAAC43310.13246479329864558No Hit
ATCTCATC38150.11668279529769865No Hit
AACTGATC32850.10047260355253998No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)