FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204047
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT340411.0624375984497105No Hit
TGCTGCTACCGTTCTC318410.9937744358931064No Hit
AAACGACCTCTTCAAG312710.975984434685259No Hit
ATGGGTTACAATCGAA296980.926890273457287No Hit
TATGCTTTGGACTGTT295990.9238004311422398No Hit
GCCTCTTGATCTGTGA282510.8817286388121024No Hit
AAAGCGGACTCGGGCT276640.8634080586208629No Hit
TCCAAAGTGCTCACGG263060.8210241610063772No Hit
TAGTGCATGAACCCGC250820.7828224742021574No Hit
TAACTCACTGGTGTCC239210.7465870506893313No Hit
TGTGGCTCTTACGATC234050.7304824180169642No Hit
GCAGGTCTGGCTGTTA227650.7105076798186792No Hit
GAAGTATGAACAGGTG214110.6682486243179329No Hit
TTCCCATCTCTTTGTT209830.65489051814783No Hit
TCACCTGACTCAATGC199490.6226188317462259No Hit
GAGCCTGACCGAGTTT198560.6197162526017876No Hit
GGAACTACTACCAAGG197670.6169385155710887No Hit
ATGTCCAGAGTCGGTC193420.6036740409862902No Hit
TGAGAATGAGCAGTTT191660.5981809879817618No Hit
CTAGAGACTAATCTGC191160.5966204615600208No Hit
TTCGTCCGACCTGTGG185860.5800788814895662No Hit
TCGTATACTGCTAATC183640.573150144177036No Hit
TCACACAGATTGGGAG179880.5614149854855438No Hit
ATTCCTAACTGCTACA175360.5473078266330051No Hit
TCCGCGAGAGAACAGC171220.5343866678609895No Hit
AGGTCGAGAAGGGAGG170510.5321707203421173No Hit
ACTGATTCTCGGCTCA170470.532045878228378No Hit
TGGCCTGGACCCGAGT167470.522682719697932No Hit
TGGACCTCTAGGGAGG164810.5143807191342699No Hit
CCATGATGATTCGGGC159950.49921240231494735No Hit
GCAATCATGGAACGTG156890.4896619806138923No Hit
TTCGATGGAAATCCTG142520.4448124512530559No Hit
ACGAGGATGGAGCTGA141810.4425965037341837No Hit
TCCCAGACTGTCTTGA136400.425711607850946No Hit
AAGGTGCGAACAGATA135740.42365171297424786No Hit
TTTCGTAACAGTACGG133800.4175968704578928No Hit
TGGTCCTTGGAGAGGG131520.4104808699747538No Hit
TCTCTCTACTCTGGAT131050.4090139751383172No Hit
TGCGGTGACCTTAGTG123310.3848570261297665No Hit
TCCTTCACTTGTCACA119210.3720607094714903No Hit
AGCCCAGTGAAAGTAC116060.36222939301452195No Hit
TTCGATGGACAGGTGA112030.3496515500552895No Hit
TGATATCCTCGATGTT111560.3481846552188529No Hit
TCGCCGAGAAGGTAAT111190.3470298656667646No Hit
AGCTGCTCTTGTCACA109740.34250433904371563No Hit
AACTCCCTGCTTCACC107880.3366991807548391No Hit
AACCCTCACGCGGTTC105140.3281474959636984No Hit
CTTTCGGTGCTTTGTT105130.32811628543526355No Hit
ATGGCCGTGATACGCT104810.32711754852534936No Hit
CCCTTGCCTCCTACGG104700.3267742327125663No Hit
TGTTTGTTGCAAGTTC97070.3029605995167986No Hit
TCGGCTTACCTCCTAC96990.30271091528932004No Hit
CTAACTGGAACCTTGA95050.29665607277296496No Hit
AAACACGCTGCAGTTT94660.295438862164007No Hit
ACCCTTGTGGGCCATC93600.292130546149916No Hit
TGGCCTTTGCGAAAGC92350.2882292300955635No Hit
TACAACGTGAGCAGGT92160.2876362300553019No Hit
AAATAGGACTCTATCT89060.2779609662405077No Hit
TCAAAGGCTCTTAGTG87970.2745590186411123No Hit
GAAGTAATGAGTCTGT87370.2726863869350231No Hit
ACAGCCGACAAACATG87130.2719373342525874No Hit
GGGGGGGGGGGGGGGG86500.26997107096119377No Hit
CAATGTGTGGTTGACT86440.26978380779058486No Hit
CTTTCGGTGAGCCATT84030.2622620704377932No Hit
TGAACTGTGTGCTGGC81400.2540537014594355No Hit
GGACATTTGGCCGCTA80770.25208743816804186No Hit
CAGCCGATGTAATAGG78420.24475296398585913No Hit
AGTGTTCGATTCGCCT77240.2410701216305504No Hit
GGCTTGCGAATACGCT71680.2237170678207904No Hit
GATAAGAACATTGGTC68750.2145723829893881No Hit
GTAAAGCCTGAAATCA67230.20982838266729548No Hit
GTGCTAATGGGAGTCA67030.20920417209859907No Hit
TTGGGAGGAATCTGTG66950.2089544878711205No Hit
TGTACTGGAAATGCTC65770.20527164551581173No Hit
CGACGTTCTTGCTACA62130.19391101316553722No Hit
GGAACATCTTTACGAC61980.1934428552390149No Hit
GGACCACTGGCTAGAG61680.1925065393859703No Hit
AGGGACCCTAATCCTG61140.19082117085049002No Hit
TCTTGAACTCGTACAT60570.18904217072970528No Hit
TAACGAGCTTACTTTC59510.18573385471561435No Hit
GGGTGAAACCGACTAT57020.17796243313534416No Hit
TACGTGTGAAACGCAG56730.17705732781073436No Hit
CGGATGAGAACAGGCA56470.17624585407142904No Hit
AACTAGCACTGCCACG55880.17440443289377466No Hit
TACCACCTGAAACATG55850.17431080130847018No Hit
AGCTTGTGAACCGTGC55140.17209485378959796No Hit
CATGATGTGCCTAGTA54450.16994132732759537No Hit
ACTTATCTGTCAGGTT54040.16866169566176775No Hit
TCGGACCGAATGTCCA52000.16229474786106446No Hit
ACGTGTTGATCTTTGT51370.1603284845696708No Hit
TGGTAGGGATGTGCTA50840.1586743265626253No Hit
AACTACGACATTGCCA50140.15648958957218792No Hit
GTAGATAGAGCTGAGA49010.15296279985905326No Hit
CTCGGATCTAGTACGG48440.1511837997382685No Hit
CGTCGTACTTGCGCCA47740.14899906274783112No Hit
GGTAGTTTGTCCAGCG46940.14650222047304548No Hit
TTTAGGGACTGGGCAA46860.14625253624556694No Hit
AACGAACCTGACACCC46840.1461901151886973No Hit
GTCCGAGTGGTCTTGA46530.14522258880721786No Hit
CCTTCAGTGCAACGCC46400.14481685193756522No Hit
AGTTTGCGAGCAGGAT46380.14475443088069556No Hit
TACCACCTGTACTTTC46170.14409900978356435No Hit
TGAGGAGTGGTCCCAG45410.14172700962251802No Hit
GCCATAATGACCGTTA44210.13798174621033962No Hit
CTTTAACTGTTCGCCT42750.13342500905885588No Hit
CAGGCCATGTCGTAGT42300.13202053527928898No Hit
CGATCGGTGCGAGGAT41760.13033516674380868No Hit
CTTACATTGGAAGGCT41090.12824406133867572No Hit
GTCCGTTACGGATGGT40740.12715169284345704No Hit
CAAAGACCTGCTTTGA40660.12690200861597847No Hit
AGAACGCGACACAGTT40540.12652748227476063No Hit
GAACCATACTTTGGGT39510.12331279784597417No Hit
GAGAAGAGATGCTACA39320.1227197978057126No Hit
TGCAATGACTCCGTAA39110.12206437670858136No Hit
AACTACGACGCTGTTA38930.12150258719675461No Hit
GGGATGTGATGCATGT38720.12084716609962338No Hit
CCGTTACTGTTGGTTG38240.11934906073475202No Hit
TCCTCATCTTTCTGGA37960.11847516593857706No Hit
GAAAGTCCTTATCTGG37930.11838153435327259No Hit
AATTCTGCTTAGCGAG37200.11610316577753074No Hit
TGTCTTCCTGAAATCA36540.1140432709008326No Hit
CCCACCTCTTGACCTA36540.1140432709008326No Hit
GGCTTAAACTGCATCA35880.11198337602413447No Hit
GCTGGACACATTGGTC35660.11129674439856845No Hit
CCTGAGGACTGACTCA35370.11039163907395864No Hit
TCGTAGAACCGCACTG35200.1098610600905667No Hit
ATAGCCAACATCTGTG35080.10948653374934887No Hit
ATGGCCGTGGGCCAAA34550.1078323757423034No Hit
ACAAGACCTAAGCTAA34460.10755148098639002No Hit
TCCGTAAGATTACGCA34430.10745784940108558No Hit
TATGGGTACCCTTTGC33100.1033068491192545No Hit
AGGTGATCTGTTAGTC32720.10212084903873132No Hit
ACTGTCCACGTTGGTT32310.1008412173729037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)