FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664402
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG255643495.94775863401995No Hit
GGCCAGGG308611.1582711617841452ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA305211.1455103246432032No Hit
GGACAGGC52520.1971174019536091No Hit
GGACACGG45420.1704697714534068No Hit
GGAAAGGG36060.1353399374418725No Hit
GGACAGCG33730.1265950108129329No Hit
CGACAGGG32770.12299195091431399No Hit
GCACAGGG32450.12179093094810767No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)