FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4513180
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA229220.5078902237446767No Hit
CGACCAGACGCGCTGT211760.4692035327640378No Hit
TAGCTTTACCCTATCC203420.4507243229829079No Hit
CCAGGACTGTAACTCG156570.34691725125078104No Hit
ATCTGTACTGGGACTA147800.32748527645695497No Hit
GTTCTCGACCGTAGAG137050.30366615113955125No Hit
CTTCCTTTGGCTCAGC135550.30034255225805306No Hit
CGATCAAACCCGGCTA129580.2871146287096903No Hit
AGACCATTGAATCCAC118020.26150075999627753No Hit
GGCAACAGAATAGCAA111940.2480291058632716No Hit
TGCTGCTACTTTCTTG109940.24359764068794063No Hit
GGGGGGGGGGGGGGGG105700.2342029345162391No Hit
ACAGCCGACTTGCGTA104260.23101227959000087No Hit
AACTTACCTCACCTAA96900.21470448774478304No Hit
CGCGTTGGAGCGGTTC90850.20129930558940703No Hit
ATCGAAAGATTTCCGC89950.1993051462605081No Hit
GTAACTGTGTGAAAGT85700.1898882827629299No Hit
CCCTGAACTAGCTTTG84210.18658684120730837No Hit
AGTGGAGTGAGTTGAA84160.1864760545779251No Hit
CAATTCGGATAACGGG81070.17962944088203883No Hit
GGGTAGAACCAGTCTA76350.16917118306825785No Hit
TTCCTAAACTAATGCC73200.16219162541711166No Hit
AAGAACCCTTAACCCA73200.16219162541711166No Hit
TTTAGTCTGAGCAATA71730.15893449851324343No Hit
CGCGATCGATATCTGG68750.15233161540200035No Hit
TGGAACACTCCTAGTA68310.15135669306342756No Hit
TCCGGGAACGTTCTCC68260.15124590643404429No Hit
GTGAAGCGATCCCAGG65080.14419987680526813No Hit
TGATAGAGAGACTGTT64140.1421170881728626No Hit
CTTTAACTGTCCGTAA64100.14202845886935597No Hit
TGCAGATACTGCCACG63920.1416296270035762No Hit
CACATCCTGAGCCTAT62850.13925879313477416No Hit
GCAACTTGAAGGTGCA62540.13857191603259786No Hit
AGGTGTGGATCATGTG62150.13770778032340833No Hit
CATAGGTACAAGGTGC61060.13529263180285298No Hit
CTATTGGGACTTGTGA60830.13478301330768994No Hit
TGCAGTATGACACCGC60140.13325415782220076No Hit
AGTCCTGCTCGAACGA60100.13316552851869412No Hit
TCTCAGAGATGCATGT59660.13219060618012132No Hit
TACGTTTACTGCACCG59500.13183608896609486No Hit
CTTAGATCTTGTCACA59220.13121568384154852No Hit
TCAAAGCACTCTGCAA58680.1300191882442092No Hit
ATTTCTGACGGGTAGT57910.12831307415170679No Hit
TGGCAATTGAGTGGTG57670.12778129833066706No Hit
ATGTACGTGTACTTTC57510.1274267811166406No Hit
GAGTGTTACACCTGCG56500.12518889120309848No Hit
TGAGGGATGTACGTTG56020.12412533956101905No Hit
AGAGTACCTTAATCGC55860.12377082234699259No Hit
TCTATGGGATGCTCAA55170.12224196686150342No Hit
GATGAATGACTCCTAC54940.12173234836634035No Hit
TGAACTGTGAACGTAA53760.11911778391289513No Hit
TTCTTAGACGTCCCGA53410.11834227750721221No Hit
GGTCGCATGGTACTAC53310.11812070424844566No Hit
GGTCTAAACTCCTACC52800.11699068062873627No Hit
TCCATTTGAAATCTGC52670.11670263539233978No Hit
CGAGTCTCTGCTACGC52410.11612654491954676No Hit
GCATGATACTTGCGTA52100.11543966781737044No Hit
GGGTGTGCTCGCAAAG51870.1149300493222074No Hit
TACTGTTTGCTTAAGC51410.11391081233188129No Hit
GGGATGTGACTGGAAG50890.11275863138629524No Hit
CACCAGGTGCGCCACA49940.11065368542801306No Hit
GTAATCTCTACACAAA49090.10877031272849742No Hit
CATTCTATGCCTAGCG49070.10872599807674412No Hit
TTGCGGTCTTTTGGGT48730.10797264899693784No Hit
CTACAACGAATCTGTG48470.10739655852414484No Hit
TCCGGGTGAACACCTA48370.1071749852653783No Hit
TCGGACCGATAGGCGA48240.10688694002898179No Hit
GGCTGACTGAAACTGA48150.10668752409609189No Hit
AGACTACTGGCCCTAG47750.10580123106102571No Hit
CTTTCGTCTCGAACGA47580.10542455652112258No Hit
CTAGCTCACGAATAGT47580.10542455652112258No Hit
CGAGTCTCTTGGAACG46830.10376275708037347No Hit
ACCAAGTTGAAGCTAA46790.10367412777686687No Hit
TGTAAGCACGCGCTGT46780.10365197045099021No Hit
CACATTACTGATGGAC46720.10351902649573029No Hit
AGCCAATTGTTATGGC46700.10347471184397697No Hit
TGCTAGTGAAGGCGTA46330.10265489078654076No Hit
CATAGTCGAAGATTCC45860.10161349647033799No Hit
ATGTCCAGACCGCTAG45760.10139192321157145No Hit
GGGCCTTGACTATCTT45690.10123682193043486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)