FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556682
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA249527797.59825429990903No Hit
GGCAAGGC93260.3647696506644158No Hit
GGCAAGAA81750.3197503639482736No Hit
GGCAAGCA41860.1637278316192628Clontech Universal Primer Mix Long (100% over 8bp)
GGCAACGA40430.1581346448248159No Hit
GGCAGGGA40410.1580564184360824No Hit
GGAAAGGA31660.12383237336516625No Hit
GCCAAGGA30020.1174178094890174RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA26740.10458868173671969No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)