FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3005966
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT326111.0848758768395916No Hit
TGCTGCTACCGTTCTC311671.0368380746821488No Hit
AAACGACCTCTTCAAG295640.9835107915392256No Hit
ATGGGTTACAATCGAA289930.9645152340379098No Hit
TATGCTTTGGACTGTT287540.956564378971685No Hit
GCAGGTCTGGCTGTTA270390.8995111721157192No Hit
AAAGCGGACTCGGGCT267200.8888989429687496No Hit
TCCAAAGTGCTCACGG253600.8436555835960886No Hit
GAAGTATGAACAGGTG240910.8014395372402748No Hit
TAGTGCATGAACCCGC239890.7980462852873254No Hit
TGTGGCTCTTACGATC225680.7507736281780965No Hit
TAACTCACTGGTGTCC223900.7448520708484394No Hit
GCCTCTTGATCTGTGA208800.6946186350743822No Hit
TTCCCATCTCTTTGTT204900.6816444364307513No Hit
TCACCTGACTCAATGC197420.6567605887757879No Hit
GGAACTACTACCAAGG192040.6388628480827794No Hit
TGAGAATGAGCAGTTT191350.636567412938137No Hit
GAAGTAATGAGTCTGT182710.6078245728660937No Hit
CTAGAGACTAATCTGC181330.603233702576809No Hit
ATGTCCAGAGTCGGTC178840.5949501757504909No Hit
TGCGGTGACCTTAGTG175950.5853359618838004No Hit
TCGTATACTGCTAATC174140.5793146030261154No Hit
TTCGTCCGACCTGTGG172720.5745906640327935No Hit
CCATGATGATTCGGGC169540.564011702061833No Hit
TCACACAGATTGGGAG169350.5633796257176561No Hit
TCCGCGAGAGAACAGC168720.5612837936290697No Hit
ATTCCTAACTGCTACA167610.5575911370920363No Hit
ACTGATTCTCGGCTCA165330.5500062209619138No Hit
AGGTCGAGAAGGGAGG162930.5420220987196794No Hit
TGGACCTCTAGGGAGG157290.5232594114504289No Hit
GCAATCATGGAACGTG156070.5192008159772932No Hit
TGGCCTGGACCCGAGT152220.506392953213709No Hit
TTCGATGGAAATCCTG137790.4583884182322754No Hit
ACGAGGATGGAGCTGA136540.4542300212311117No Hit
AAGGTGCGAACAGATA132400.4404574103632576No Hit
TTTCGTAACAGTACGG131410.437163959938336No Hit
TGGTCCTTGGAGAGGG128140.42628559338329175No Hit
TCCCAGACTGTCTTGA126530.4209295780457929No Hit
TCTCTCTACTCTGGAT124180.4131117916836052No Hit
GCGGTTCGAGTACGTT122040.40599261601761294No Hit
TCGCCGAGAAGGTAAT111500.3709290125038008No Hit
TTCGATGGACAGGTGA111180.3698644628715029No Hit
TCCTTCACTTGTCACA110330.3670367529107116No Hit
TGATATCCTCGATGTT108930.3623793482694082No Hit
AGCTGCTCTTGTCACA108360.3604831192368776No Hit
AACTCCCTGCTTCACC105300.3503033633780289No Hit
CTTTCGGTGCTTTGTT104800.34864000457756344No Hit
AGCCCAGTGAAAGTAC101420.3373956990864168No Hit
TCGGCTTACCTCCTAC98050.3261846607712795No Hit
GGGGGGGGGGGGGGGG96350.3205292408496969No Hit
AACCCTCACGCGGTTC96280.3202963706176317No Hit
CCCTTGCCTCCTACGG96100.31969756144946415No Hit
ATGGCCGTGATACGCT95900.31903221792927794No Hit
TGTTTGTTGCAAGTTC94310.31374273694379773No Hit
AAACACGCTGCAGTTT92440.3075217750300569No Hit
CTAACTGGAACCTTGA88770.2953127214346403No Hit
CAATGTGTGGTTGACT86160.2866299884962105No Hit
AAATAGGACTCTATCT85610.28480029381569855No Hit
TACAACGTGAGCAGGT85480.2843678205275775No Hit
ACCCTTGTGGGCCATC84980.282704461727112No Hit
ACAGCCGACAAACATG84260.2803092250544417No Hit
TGGCCTTTGCGAAAGC83890.27907833954209726No Hit
TCAAAGGCTCTTAGTG82560.27465380513285914No Hit
GGACATTTGGCCGCTA81200.27012946919559305No Hit
CTTTCGGTGAGCCATT79120.26320989658565663No Hit
TGAACTGTGTGCTGGC77660.2583528888882975No Hit
GAGGTTGGAGGAATAT75140.24996956053395147No Hit
CAGCCGATGTAATAGG73630.24494621695654575No Hit
AGTGTTCGATTCGCCT72230.2402888123152424No Hit
GATAAGAACATTGGTC70900.23586427790600428No Hit
GGCTTGCGAATACGCT69100.22987618622432854No Hit
CGACGTTCTTGCTACA66860.22242433879824322No Hit
GTGCTAATGGGAGTCA66450.22106038458186156No Hit
GTAAAGCCTGAAATCA65690.21853207920515405No Hit
TTGGGAGGAATCTGTG62620.208319056170296No Hit
TGTACTGGAAATGCTC62580.20818598746625877No Hit
GGAACATCTTTACGAC60160.20013533087200586No Hit
GGGTGAAACCGACTAT58850.19577733081478635No Hit
AGGGACCCTAATCCTG57620.19168546816564128No Hit
TACCACCTGAAACATG56030.18639598718016107No Hit
TAACGAGCTTACTTTC55490.18459955967565833No Hit
TACGTGTGAAACGCAG55380.18423362073955596No Hit
TCTTGAACTCGTACAT55190.18360154439537904No Hit
AGCTTGTGAACCGTGC54920.18270333064312771No Hit
CGGATGAGAACAGGCA54760.18217105582697873No Hit
ACTTATCTGTCAGGTT53430.17774652141774058No Hit
CATGATGTGCCTAGTA52210.17368792594460483No Hit
AACTAGCACTGCCACG49930.16610300981448228No Hit
ACGTGTTGATCTTTGT48430.16111293341308583No Hit
TGGTAGGGATGTGCTA46800.1556903837235684No Hit
AACTACGACATTGCCA46510.15472563561929842No Hit
GTAGATAGAGCTGAGA46480.1546258340912705No Hit
AACGAACCTGACACCC46340.15416009362714014No Hit
TCGGACCGAATGTCCA46100.15336168140291673No Hit
CGTCGTACTTGCGCCA46080.1532951470508981No Hit
CTCGGATCTAGTACGG44850.14920328440175304No Hit
CCTTCAGTGCAACGCC44370.14760645995330618No Hit
GGTAGTTTGTCCAGCG44060.1465751774970176No Hit
AGTTTGCGAGCAGGAT43470.14461241411246833No Hit
TTTAGGGACTGGGCAA43320.1441134064723287No Hit
CTTTAACTGTTCGCCT43060.14324845989608664No Hit
TACCACCTGTACTTTC43040.14318192554406803No Hit
TGAGGAGTGGTCCCAG41600.13839145219872745No Hit
AGAACGCGACACAGTT39840.132536429221089No Hit
GAGAAGAGATGCTACA39520.1314718795887911No Hit
GAAAGTCCTTATCTGG39430.1311724750047073No Hit
CTTACATTGGAAGGCT39030.12984178796433493No Hit
CGATCGGTGCGAGGAT39010.12977525361231632No Hit
GCCATAATGACCGTTA38870.12930951314818598No Hit
GGGATGTGATGCATGT38860.12927624597217666No Hit
GGACCACTGGCTAGAG38720.12881050550804632No Hit
CAAAGACCTGCTTTGA37290.12405329933871506No Hit
CAGGCCATGTCGTAGT37260.12395349781068715No Hit
GCTGGACACATTGGTC36400.12109252067388653No Hit
GAAGGACACCCAGCTG36160.1202941084496631No Hit
TGCAATGACTCCGTAA35860.11929609316938383No Hit
AATTCTGCTTAGCGAG35710.11879708552924417No Hit
CCGTTACTGTTGGTTG35560.11829807788910453No Hit
AACTACGACGCTGTTA35020.11650165038460181No Hit
TCCTCATCTTTCTGGA34950.11626878015253667No Hit
GGCTTAAACTGCATCA33650.11194404727132641No Hit
CCTGAGGACTGACTCA33290.11074642893499129No Hit
TGTCTTCCTGAAATCA33230.11054682587893541No Hit
TCGTAGAACCGCACTG33060.10998128388677716No Hit
GCGTTTAGAAGTCTAC32890.1094157418946189No Hit
CCCACCTCTTGACCTA32420.10785218462218135No Hit
ATAGCCAACATCTGTG32180.10705377239795794No Hit
ATGGCCGTGGGCCAAA31930.10622209299772518No Hit
ACAAGACCTAAGCTAA31900.10612229146969726No Hit
TCCGTAAGATTACGCA31840.1059226884136414No Hit
TATGGGTACCCTTTGC31600.10512427618941798No Hit
AGGTGATCTGTTAGTC30750.10229656622862666No Hit
GCGTTAATGGTACGTT30490.10143161965238462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)