FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876666
Sequences flagged as poor quality0
Sequence length34
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30460.34745273570550245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG458.704048E-418.6635865
GTAAGGC652.9889634E-517.2230073
TAAGGCA1055.436086E-714.6608974
CAGTACA1159.535688E-814.6029484
TTAGACT903.0427196E-513.99449354
CTTAGAC801.7596968E-413.9936923
CTATACT852.9397284E-413.1712874
TAGGCAG1809.786163E-1012.442395
CCTATAC904.7648876E-412.438843
ATTAGGT850.004390644411.5242193
GAACAGT1000.001149406211.2000686
TCAAGAC1401.3650068E-510.9950463
TATGCCT900.006718079610.8846064
GTCCAGA1307.772228E-510.7668381
CTAAGAC1050.001733702710.6618613
TAGCCCT1100.002551189610.1778134
TCTAGGC1256.487518E-410.07545953
GTGTAGG1401.6456499E-49.9977791
GCTCATT1150.0036676129.74141511
GTGTTAG1150.00368149049.7369661
Produced by FastQC (version 0.11.5)