FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18458074
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC19250.011.85347322
CTTATAC25400.010.3655061
TATACTG22050.09.8442985
CACGAGA23950.09.64419521
TATACAC41900.09.4921283
CCCACGA24650.09.4271919
ATCTCCG24350.09.19842110
TAGACCG5700.09.0905165
CTAGTAC12150.08.8751113
TCTCCGA26350.08.81900711
TAGACTG25250.08.7630925
TAGGACA24900.08.6612534
TAGACAG32750.08.2529215
CCACGAG29800.08.17375720
GTCTAGG26800.08.0995941
TTATACA36900.08.0837012
TACACTG32550.08.0024615
GACTCTA29600.07.945312526
GTCCTAG28250.07.8821571
CTAAGAC22350.07.7696863
Produced by FastQC (version 0.11.5)