FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5411211
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT526124197.228531654005No Hit
CTAGCTGA305100.563829427460877No Hit
CTAGCAGT104630.19335782692635717No Hit
CAAGCTGT95360.17622672632798833No Hit
CTCGCTGT94790.17517335768278117No Hit
CTAGATGT89630.16563759942090597No Hit
CTAGCTAT84250.15569527782228412RNA PCR Primer, Index 38 (100% over 8bp)
CTAGCTGG78710.1454572737969375No Hit
CTAGCTCT77630.143461417416545No Hit
ATAGCTGT74740.13812065358382808No Hit
CTACCTGT74610.13788041161211417No Hit
CTAGCGGT65440.12093411253044836No Hit
CTAGCTTT57470.10620543164921864No Hit
CTTGCTGT54530.10077226705815019No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)