FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126832
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC303536697.07480286756692Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC239150.7648316251080967No Hit
TGTAGATA153520.490976170130023No Hit
AGTAGATC59970.19179156411345413No Hit
TGTAGAAC55910.17880717608109423Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC55580.1777517947878236No Hit
TGTACATC48660.1556207688804515No Hit
TCTAGATC34770.11119881080915124ABI Dynabead EcoP Oligo (100% over 8bp)
TATAGATC33550.10729709814918102No Hit
TTTAGATC31820.10176434167233801No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)