FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4458579
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA439701898.61926860553552No Hit
CCCAACAA95200.21352094467766525RNA PCR Primer, Index 36 (100% over 8bp)
GCCAAAAA69060.15489239957394496No Hit
ACCAACAA67640.1517075283403075No Hit
GACAACAA56020.1256454130340631No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)