FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17983643
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT4600.09.7390956
TAGGACA20000.08.6099684
TACACTG31650.08.5813275
TACACCG7100.08.4788425
TATACTG19000.08.4736535
TCTATAC13900.08.3601693
TACCGTC5850.08.1351617
TAGACTG24800.08.1290035
TAGACAG29900.08.0534825
GTATAGA15550.08.0147041
CCTACAC24450.07.9595353
TAGTACT15100.07.88076544
CCTATAC13550.07.85282853
CTATACT19150.07.82242544
TCTAGAC19350.07.8143813
TAGGCCG13850.07.7833655
GTGTAAG24300.07.7795671
TTATACT22050.07.6825124
GTGTAGG26150.07.6575931
GTAGGAC16150.07.62890053
Produced by FastQC (version 0.11.5)