FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17270009
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG31600.09.38983255
TATACTG20350.08.8034865
TATACCG3201.2660166E-98.7476045
TAGGACT22150.08.2121914
GTATTAG16200.08.20469951
GTCTTAG20450.08.0047291
GCTACAC22950.07.92519043
ATACCGT4801.8189894E-127.87391766
GTCTAGA19750.07.7925521
TAGACTG22000.07.7615115
CTAGTAC9250.07.71396163
TAGACCG7100.07.68803555
CTATACT16650.07.6474484
CTACACT29500.07.63648034
CTTATAC13450.07.5936343
CTAGGAC23800.07.52457673
AGGACTG40050.07.4786015
GTCCTAG23600.07.46986341
GTCCTAA18000.07.46195841
ACACCGT9950.07.4562616
Produced by FastQC (version 0.11.5)