FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19527638
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG22700.09.8679425
GTATTAG20150.08.6159571
TACACTG32600.08.3313725
CTACACT31450.08.3243024
TCTATAC16500.08.2301553
ATACTGT32000.08.2251466
GTACCGT8200.08.1952686
GTACTAG13550.08.0595761
TAGACTG22850.07.96507025
GTGTAGG26900.07.9633551
GTATAGG16550.07.95219041
GTATAGA19200.07.8755231
ATACCGT4709.094947E-127.7448196
TAGACAG34100.07.71855355
GTTCTAG25550.07.6717431
CTATACT21950.07.6537384
TCTAGAC23100.07.5756213
TACACCG9100.07.5385385
ACACCGT11350.07.5243636
TACCCCG16050.07.50163365
Produced by FastQC (version 0.11.5)