FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004949440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394504
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC262470.5972687702639479No Hit
CTCCTAGTGAACGTAA228950.5209916750559336No Hit
CGACCAGACGCGCTGT219400.49925998474458094No Hit
TAGCTTTACCCTATCC199530.454044415478971No Hit
CCAGGACTGTAACTCG159330.3625665149013404No Hit
ATCTGTACTGGGACTA143680.3269538496267156No Hit
CTTCCTTTGGCTCAGC137210.3122309138869825No Hit
GTTCTCGACCGTAGAG136550.31072903790734974No Hit
CGATCAAACCCGGCTA128340.29204661094858486No Hit
NNNNNNNNNNNNNNNN127310.28970277419249135No Hit
AGACCATTGAATCCAC125230.28496958928698213No Hit
TGCTGCTACTTTCTTG114570.2607120166462472No Hit
GGGGGGGGGGGGGGGG107460.24453271632020357No Hit
ACAGCCGACTTGCGTA105160.2392989060881501No Hit
AACTTACCTCACCTAA95730.21784028413673076No Hit
CGCGTTGGAGCGGTTC92890.21137766628497776No Hit
ATCGAAAGATTTCCGC88330.2010010686075152No Hit
CCCTGAACTAGCTTTG85620.19483427481235654No Hit
GTAACTGTGTGAAAGT85030.19349168870935152No Hit
GGCAACAGAATAGCAA83750.19057895953673043No Hit
AGTGGAGTGAGTTGAA82350.18739316200417613No Hit
CAATTCGGATAACGGG79970.18197730619883382No Hit
GGGTAGAACCAGTCTA74770.1701443439350607No Hit
AAGAACCCTTAACCCA73770.16786877426895047No Hit
TTCCTAAACTAATGCC73610.16750468312237288No Hit
TCCGGGAACGTTCTCC70760.16101930957395874No Hit
CGCGATCGATATCTGG70170.1596767234709537No Hit
GTGAAGCGATCCCAGG69740.15869822851452633No Hit
TTTAGTCTGAGCAATA69090.1572191082315547No Hit
TGGAACACTCCTAGTA68620.1561495904884829No Hit
TGCAGATACTGCCACG67780.15423811196895032No Hit
CTTTAACTGTCCGTAA63880.14536339027112047No Hit
TGATAGAGAGACTGTT63850.14529512318113716No Hit
AGTCCTGCTCGAACGA63260.14395253707813213No Hit
CACATCCTGAGCCTAT63220.14386151429148772No Hit
TGCAGTATGACACCGC61880.14081225093890004No Hit
AGGTGTGGATCATGTG59760.1359880432467464No Hit
TACGTTTACTGCACCG59510.13541915083021883No Hit
TCTCAGAGATGCATGT59490.13537363943689665No Hit
GCAACTTGAAGGTGCA58990.13423585460384152No Hit
ATTTCTGACGGGTAGT58760.13371247358063618No Hit
GAGTGTTACACCTGCG58640.13343940522070297No Hit
CTATTGGGACTTGTGA58610.13337113813071966No Hit
CATAGGTACAAGGTGC58470.13305255837746421No Hit
TCAAAGCACTCTGCAA58300.1326657115342255No Hit
CTTAGATCTTGTCACA57800.13152792670117036No Hit
CGAGTCTCTGCTACGC56790.12922960133839906No Hit
AGAGTACCTTAATCGC56410.1283648848652772No Hit
TGGCAATTGAGTGGTG56120.1277049696621052No Hit
TCTATGGGATGCTCAA55990.1274091456055109No Hit
TGAACTGTGAACGTAA55750.12686300888564442No Hit
ATGTACGTGTACTTTC55260.12574797974925042No Hit
TTCTTAGACGTCCCGA54950.12504255315275625No Hit
TGAGGGATGTACGTTG54310.12358618856644574No Hit
GGTCTAAACTCCTACC54250.12344965438647912No Hit
GGTCGCATGGTACTAC52930.12044590242721363No Hit
GGGTGTGCTCGCAAAG52470.11939914038080293No Hit
TACTGTTTGCTTAAGC51780.11782899731118687No Hit
GGGATGTGACTGGAAG51640.11751041755793144No Hit
TCCATTTGAAATCTGC51520.11723734919799823No Hit
CTCAATTTGGAATCTC50870.11575822891502659No Hit
CTACAACGAATCTGTG50500.1149162681385658No Hit
CACCAGGTGCGCCACA50170.11416533014874944No Hit
GTAATCTCTACACAAA49810.11334612506894977No Hit
TTGCGGTCTTTTGGGT49700.11309581240567763No Hit
CTAGCTCACGAATAGT49630.11293652252904991No Hit
CATTCTATGCCTAGCG49170.11188976048263923No Hit
CTTTCGTCTCGAACGA48820.11109331109950066No Hit
TCCGGGTGAACACCTA48510.11038788450300648No Hit
AGACTACTGGCCCTAG48310.10993277056978443No Hit
TCGGACCGATAGGCGA48290.10988725917646225No Hit
CGAGTCTCTTGGAACG48020.10927285536661248No Hit
TGCTAGTGAAGGCGTA47760.10868120725342383No Hit
CACATTACTGATGGAC47540.10818058192687957No Hit
TGTAAGCACGCGCTGT47320.10767995660033533No Hit
AGCCAATTGTTATGGC47180.10736137684707989No Hit
ACCAAGTTGAAGCTAA47150.10729310975709659No Hit
TGCGCAGACATCAGGG46800.10649666037395801No Hit
CATAGTCGAAGATTCC46100.10490376160768088No Hit
ATGTCCAGACCGCTAG45770.10415282361786449No Hit
AGGCGATGACCTGCGA45760.10413006792120338No Hit
GGCTGACTGAAACTGA45370.1032425957514204No Hit
ATAGCCAACCGTTCTC45350.10319708435809821No Hit
TAGTACCGAACGAGCA45070.10255992485158735No Hit
ACCTCAGCTAATCCGT44870.1021048109183653No Hit
GGGCCTTGACTATCTT44640.10158142989515995No Hit
ACGAGGATGAACTAGA44550.10137662862521003No Hit
CTAACAGTGCATGATC44440.10112631596193793No Hit
TCATCATTGGACTCGC44250.10069395772537697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)