FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636648
Sequences flagged as poor quality0
Sequence length8
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG346212795.20104777806375No Hit
GGCCAGGG633251.7413013302359757ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA416971.1465778376130986No Hit
GGACAGGC76210.20956111232101649No Hit
GGACACGG63670.17507880883714894No Hit
GGTCAGGG58990.16220981519245195No Hit
GGAAAGGG53640.14749846561998853No Hit
CGACAGGG50260.13820419243215182No Hit
GGACAGCG46000.12649010847351735No Hit
GGGCAGGG41470.11403358257384272No Hit
GCACAGGG38300.10531676422903728No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)