FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004949461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4457720
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG436406097.89892590831187No Hit
TAATGGTG101140.22688728767172456No Hit
TATTGGTA100970.22650592679665837No Hit
AATTGGTG81770.1834345809068313No Hit
TATTGGGG71020.15931911380705832No Hit
TATTGGTC65450.14682393690047826No Hit
TATTGGAG64760.1452760604075626No Hit
TATTGGTT59220.13284818247893543No Hit
TATAGGTG56690.12717263533824466RNA PCR Primer, Index 39 (100% over 8bp)
TATTGCTG55470.12443581023482858No Hit
TATTCGTG54030.12120545929309152No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)