FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17019049
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC17150.010.9409622
CTTATAC25050.09.7804661
ATCTCCG21250.09.22195610
TATACAC38750.09.175563
TCTCCGA22600.09.10464311
TACACCG8150.09.1027425
CCCACGA22050.08.88862719
TACACTG32150.08.8383065
GTATAGA16000.08.8375251
CACGAGA21700.08.77491521
GTATTAG18250.08.3616681
TAGACAG31250.08.3313795
TATACTG22200.08.2598525
TAGACTG24750.08.2006225
GTACTAG11450.08.1921631
TTATACA33650.08.0288682
TAGACGG6800.08.0280435
TAGGACA23300.07.9900544
GTCTTAC15950.07.9874831
ATACCGT4401.6370905E-117.95321276
Produced by FastQC (version 0.11.5)