FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004949471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17019049
Sequences flagged as poor quality0
Sequence length34
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252000.1480693780245888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG32250.08.8541285
TATACTG19400.08.6581465
TAGGTCG3601.4733814E-108.5539275
GTGTAGA22850.08.2113061
CCTATAC13200.08.1637443
TACGCTG6650.07.99847755
TCTACAC22500.07.8993943
GTATTAG19050.07.79120251
TAGACAG28400.07.787255
GTGTTAG24200.07.6953771
CTTAGAC17100.07.69315153
TCTATAC14350.07.6070333
TTACACT24100.07.54971934
GTCTAGA21000.07.53447151
GTCCTAG26250.07.52113631
CCTACAC26350.07.43566753
ACCGGTC7350.07.4293228
CTATACT18300.07.41865444
TAGACCG5850.07.4173925
TGTACCG6450.07.37844045
Produced by FastQC (version 0.11.5)