FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004949477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004949477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3144535
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC306333797.41780581230611Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA239540.7617660480802408No Hit
ATCAGATC73870.23491549624984298Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGCTC65120.20708944247718664No Hit
ATATGATC56280.17897717786572578No Hit
ATCTGAAC46260.14711237114549527No Hit
ATCTCATC43920.13967088933657917No Hit
ATCTGAGC39750.12640978713863893No Hit
AACTGATC38360.12198941973932552No Hit
AGCTGATC34950.11114520906906745No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)